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We describe a new subfamily of satellite III DNA (pTRS-63), which, by a combination of in situ hybridization to human metaphase chromosomes and analysis of a panel of somatic cell hybrids, is shown to be specific for human chromosome 14. This DNA has a basic 5-bp repeating unit of diverged GGAAT which is tandemly repeated and organized into either one of two distinct higher-order structures of 5 kb (designated the "L" form) or 4.8 kb (designated the "S" form). In addition, a third (Z) form, representing no detectable levels of this satellite III subfamily, is found. Results from five somatic cell hybrid lines and from a number of informative human individuals suggest that, on any one chromosome 14, only one of the three forms may exist. Subchromosomally, this sequence has been mapped to the p11 region and is distal to the domain occupied by another previously described satellite III subfamily (pTRS-47) found on chromosome 14. The pTRS-63 sequence described adds to the understanding of the structural organization of the short arm of human chromosome 14 and should be useful for the investigation of the molecular etiology of the frequently occurring t(13q14q) and t(14q21q) Robertsonian translocations.
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
July 1979,
Nucleic acids research,
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
January 1992,
Cytogenetics and cell genetics,
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
February 1979,
Chromosoma,
K H Choo, and
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B Vissel, and
P Kalitsis
November 1974,
Experientia,
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
May 1985,
American journal of human genetics,
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
April 1990,
American journal of human genetics,
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
January 1988,
Doklady Akademii nauk SSSR,
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
August 1986,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
April 1996,
Genomics,
K H Choo, and
E Earle, and
B Vissel, and
P Kalitsis
August 1988,
Genomics,
