Biomaterial Database

[Primary culture of strial marginal cells of guinea pig cochlea]. 2002

Min Fu, and Weijia Kong

Department of Otolaryngology, Guangdong Provincial Peoples Hospital, Guangzhou 510080.

OBJECTIVE For further study of the function of marginal cells, primary cultures of marginal cells of guinea pig were established. METHODS Tissue of stria vascularis was transferred from the cochlea to a cell-cultured dish using the explants culture technique. Explants were cultivated at 37 degrees C in a 5% CO2 incubator and examined daily by interted microscopy. The culture medium was changed twice a week. Antibodies of cytokeratin and vimentin were used to examine the cultured cells by immunohistochemical methods. The ultrastructure of the cultured cells was observed by making the electron microscopy specimens. RESULTS Explants were successfully cultivated for 4 weeks. Two different cells, hexagonal-shaped cells and spindle-shaped cells were observed. Cytokeratin and vimentin staining occurred in cytoplasm of hexagonal-shaped cells, whereas spindle-shaped cells gave a negative cytokeratin staining but a positive vimentin staining. Electron microscopy of the explants revealed that hexagonal-shaped cells have the epithelial morphologic characteristics such as tight junctions and desmosomes. CONCLUSIONS Primary culture of marginal cells by explants culture technique provides an optimal model for investigation of the function of the marginal cells.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003051	Cochlea	The part of the inner ear (LABYRINTH) that is concerned with hearing. It forms the anterior part of the labyrinth, as a snail-like structure that is situated almost horizontally anterior to the VESTIBULAR LABYRINTH.	Cochleas
D005260	Female		Females
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013316	Stria Vascularis	A layer of stratified EPITHELIUM forming the endolymphatic border of the cochlear duct at the lateral wall of the cochlea. Stria vascularis contains primarily three cell types (marginal, intermediate, and basal), and capillaries. The marginal cells directly facing the ENDOLYMPH are important in producing ion gradients and endochoclear potential.	Vascularis, Stria
D018929	Cell Culture Techniques	Methods for maintaining or growing CELLS in vitro.	Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell