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BACKGROUND Recent evidence indicates that nitric oxide (NO) has a crucial role in hepatic ischemia-reperfusion (I/R) injury. However, little is known about how I/R influences the gene expression of inducible nitric oxide synthase (iNOS) in hepatocytes. Under inflammatory conditions, we compared the induction of iNOS in hepatocytes isolated from normal and I/R-treated rats. METHODS Hepatocytes were isolated using the collagenase perfusion method from rats treated with I/R (30-minute ischemia of middle and left lobes, followed by 3-hour reperfusion) or sham operation (control): Primary cultures of rat hepatocytes were incubated with an inflammatory cytokine, interleukin-1beta (IL-1beta), to compare the iNOS induction/NO production between the 2 groups. RESULTS Both control and I/R groups had no production of nitrite (a stable metabolite of NO) in the absence of IL-1beta. In the control group, IL-1beta stimulated dose- and time-dependent production of NO. The I/R group showed more than 2-fold increased levels of NO production. Western and Northern blot analyses revealed that the I/R group also showed increased levels of iNOS protein and its messenger RNA. CONCLUSIONS These results suggest that I/R directly affects the inducibility of the iNOS gene in hepatocytes by IL-1beta. Increased NO may be associated with protective or toxic effects in hepatic I/R injury.
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
November 1996,
Experimental eye research,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
March 2002,
Kidney international,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
February 2013,
Canadian journal of surgery. Journal canadien de chirurgie,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
May 2018,
Sleep & breathing = Schlaf & Atmung,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
September 2007,
The Journal of trauma,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
May 2011,
Anesthesiology,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
January 1999,
Life sciences,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
November 2000,
Transplantation proceedings,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
March 2005,
Acta pharmacologica Sinica,
H Yanagida, and
M Kaibori, and
M Yamada, and
K Habara, and
N Yokoigawa, and
A-H Kwon, and
Y Kamiyama, and
T Okumura
September 1999,
The American journal of physiology,
