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The need to develop a blood substitute is now urgent because of the increasing concern over blood-transmitted viral and bacterial pathogens. Cell-free haemoglobin solutions and human haemoglobin synthesized in Escherichia coli and Saccharomyces cerevisiae have been investigated as potential oxygen-carrying substitutes for red blood cells. But these haemoglobins cannot be used as a blood substitute because (1) the oxygen affinity in the absence of 2,3-bisphosphoglycerate is too high to allow unloading of enough oxygen in the tissues, and (2) they dissociate into alpha beta dimers that are cleared rapidly by renal filtration, which can result in long-term kidney damage. We have produced a human haemoglobin using an expression vector containing one gene encoding a mutant beta-globin with decreased oxygen affinity and one duplicated, tandemly fused alpha-globin gene. Fusion of the two alpha-globin subunits increases the half-life of this haemoglobin molecule in vivo by preventing its dissociation into alpha beta dimers and therefore also eliminates renal toxicity.
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
January 1987,
Advances in experimental medicine and biology,
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
October 1988,
Journal of biochemical and biophysical methods,
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
January 1992,
The National medical journal of India,
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
January 1989,
Transfusion,
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
January 1993,
American journal of hematology,
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
October 1943,
Science (New York, N.Y.),
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
July 1940,
British medical journal,
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
March 1999,
BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy,
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
August 2014,
Zhongguo shi yan xue ye xue za zhi,
D Looker, and
D Abbott-Brown, and
P Cozart, and
S Durfee, and
S Hoffman, and
A J Mathews, and
J Miller-Roehrich, and
S Shoemaker, and
S Trimble, and
G Fermi
February 1976,
Journal of the American Veterinary Medical Association,
