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Two lipoxygenase isoenzymes (linoleate: oxygen oxidoreductase, EC 1.13.11.12) present in the embryo of germinating barley seed have been purified to homogeneity and characterized. Both isoenzymes are monomeric proteins with a molecular mass of approx. 90 kDa and crossreact on Western blots with antibodies raised against pea lipoxygenase. They have an apparent Km of approx. 16 microM for linoleic acid. The isoenzymes differ in the product formed upon incubation with linoleic acid. One of the isoenzymes (lipoxygenase 1) solely forms the 9-HPOD as a product whereas the 13-HPOD is the major product formed by the other isoenzyme (lipoxygenase 2). Lipoxygenase 1 shows a pH-optimum of 6.5, is active in a broad pH range and has an isoelectric point of 5.2-5.3. Lipoxygenase 2 has the same pH optimum, but is active in a narrow pH range and has a significantly higher pI, namely 6.8-6.9. The occurrence of two isoenzymes was confirmed by peptide analysis of the proteins. Amino acid sequence data obtained from proteolytic fragments of lipoxygenase 1 show up to 50% identity with other plant lipoxygenases.
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
January 1978,
Planta,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
June 1996,
Plant physiology,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
January 2015,
Applied biochemistry and biotechnology,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
November 1963,
Archives internationales de physiologie et de biochimie,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
April 2000,
Journal of agricultural and food chemistry,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
April 1977,
Plant physiology,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
February 2006,
Journal of agricultural and food chemistry,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
March 1991,
FEBS letters,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
June 2016,
3 Biotech,
A Doderer, and
I Kokkelink, and
S van der Veen, and
B E Valk, and
A W Schram, and
A C Douma
December 1985,
Archives of biochemistry and biophysics,
