Furin-mediated cleavage of the feline foamy virus Env leader protein. 2004

Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
Abt. Genomveränderung und Carcinogenese, Forschungsschwerpunkt Infektion und Krebs, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany.

The molecular biology of spuma or foamy retroviruses is different from that of the other members of the Retroviridae. Among the distinguishing features, the N-terminal domain of the foamy virus Env glycoprotein, the 16-kDa Env leader protein Elp, is a component of released, infectious virions and is required for particle budding. The transmembrane protein Elp specifically interacts with N-terminal Gag sequences during morphogenesis. In this study, we investigate the mechanism of Elp release from the Env precursor protein. By a combination of genetic, biochemical, and biophysical methods, we show that the feline foamy virus (FFV) Elp is released by a cellular furin-like protease, most likely furin itself, generating an Elp protein consisting of 127 amino acid residues. The cleavage site fully conforms to the rules for an optimal furin site. Proteolytic processing at the furin cleavage site is required for full infectivity of FFV. However, utilization of other furin proteases and/or cleavage at a suboptimal signal peptidase cleavage site can partially rescue virus viability. In addition, we show that FFV Elp carries an N-linked oligosaccharide that is not conserved among the known foamy viruses.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011498 Protein Precursors Precursors, Protein
D002415 Cats The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801) Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014771 Virion The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos. Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles
D015686 Gene Products, env Retroviral proteins, often glycosylated, coded by the envelope (env) gene. They are usually synthesized as protein precursors (POLYPROTEINS) and later cleaved into the final viral envelope glycoproteins by a viral protease. env Gene Products,env Polyproteins,env Protein,env Antigens,env Glycoproteins,env Polyprotein,Antigens, env,Polyprotein, env,Polyproteins, env
D016092 Spumavirus Genus of non-oncogenic retroviruses which establish persistent infections in many animal species but are considered non-pathogenic. Its species have been isolated from primates (including humans), cattle, cats, hamsters, horses, and sea lions. Spumaviruses have a foamy or lace-like appearance and are often accompanied by syncytium formation. SIMIAN FOAMY VIRUS is the type species. African green monkey simian foamy virus,Bovine Syncytial Virus,Bovine foamy virus,Feline Syncytium-Forming Virus,Feline foamy virus,Foamy Virus,Macaque simian foamy virus,Simian foamy virus 1,Simian foamy virus 3,Spumavirinae,Spumaviruses,Bovine Syncytial Viruses,Bovine foamy viruses,Feline Syncytium Forming Virus,Feline Syncytium-Forming Viruses,Feline foamy viruses,Foamy Viruses,Syncytial Virus, Bovine,Syncytial Viruses, Bovine,Syncytium-Forming Virus, Feline,Syncytium-Forming Viruses, Feline,foamy virus, Bovine,foamy viruses, Bovine,foamy viruses, Feline,virus, Feline foamy,viruses, Bovine foamy,viruses, Feline foamy

Related Publications

Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
December 2001, The Journal of general virology,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
September 2001, Journal of virology,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
January 2000, Virology,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
December 2004, Journal of virology,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
April 2014, Proceedings of the National Academy of Sciences of the United States of America,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
November 2011, Viruses,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
March 2000, Journal of virology,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
June 2006, Journal of virology,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
April 2015, The Journal of general virology,
Verena Geiselhart, and Patrizia Bastone, and Tore Kempf, and Martina Schnölzer, and Martin Löchelt
October 2004, The Journal of general virology,
Copied contents to your clipboard!