Short report: evaluation of a simple and inexpensive photometric device for the measurement of hemoglobin. 2004

Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
Medical Research Unit, Albert Schweitzer Hospital, BP 118, Lambaréné, Gabon. steffen.borman@uni-tuebingen.de

We have evaluated the accuracy of a simple and inexpensive photometric device (DHT) for the estimation of the blood concentration of hemoglobin by comparison with an automated, high-resolution, flow cytometry-based hematology analyzer (CellDyn 3000) and a centrifugal quantitative buffy coat hematology system (QBC I). We have analyzed the hemoglobin values of 163 individual blood samples. Bland-Altman analysis showed that the methods agreed only poorly: mean differences were 1.0 g/dL with limits of agreement (LOA) of -1.2 g/dL to 3.2 g/dL for the comparison of DHT and CellDyn measurements, 0.5 g/dL with LOA of -2.0 g/dL to 3.0 g/dL for the comparison of DHT with QBC measurements, and 0.5 g/dL with LOA of -1.1 g/dL to 2.1 g/dL for the comparison of QBC with CellDyn measurements. We conclude that the poor agreement of the DHT with the CellDyn does not permit the use of the DHT for critical hemoglobin measurements, particularly in transfusion services.

UI MeSH Term Description Entries
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D003124 Colorimetry Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006452 Hemoglobinometry Measurement of hemoglobin concentration in blood. Hemoglobinometries
D006454 Hemoglobins The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements. Eryhem,Ferrous Hemoglobin,Hemoglobin,Hemoglobin, Ferrous
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D019095 Point-of-Care Systems Laboratory and other services provided to patients at the bedside. These include diagnostic and laboratory testing using automated information entry. Bedside Computing,Point of Care Technology,Bedside Technology,Point-of-Care,Bedside Technologies,Computing, Bedside,Point of Care,Point of Care Systems,Point-of-Care System,Systems, Point-of-Care,Technologies, Bedside,Technology, Bedside

Related Publications

Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
February 1997, Masui. The Japanese journal of anesthesiology,
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
March 1951, American journal of clinical pathology,
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
February 1987, Surgery, gynecology & obstetrics,
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
March 2014, Journal of microbiological methods,
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
September 1977, Lancet (London, England),
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
May 1967, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
May 1985, Annals of plastic surgery,
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
May 1972, The New England journal of medicine,
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
August 2002, Masui. The Japanese journal of anesthesiology,
Steffen Borrmann, and Sunny Oyakhirome, and Gilbert Esser, and Cordula Trinkle, and Saadou Issifou, and Martin P Grobusch, and Sanjeev Krishna, and Peter G Kremsner
March 1998, Medical & biological engineering & computing,
Copied contents to your clipboard!