BIOMAT Database Logo BIOMAT Database Logo

Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case. 2004

Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Laboratoire de Spectroscopie Moléculaire de Surface, University of Namur, rue de Bruxelles, 61, 5000 Namur, Belgium. laurent.dreesen@fundp.ac.be

Infrared/visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derived vitamin (biocytin) adsorbed on a calcium fluoride substrate. The specificity of the process is tested by replacing avidin with bovine serum albumin or presaturated avidin. The SFG spectroscopy shows drastic modifications in the CH and NH spectral ranges only upon exposure of the biocytin film to avidin. The comparison of the SFG data with Fourier transform infrared reflection absorption spectra (FT-IRRAS) in the same spectral ranges illustrates the advantages of nonlinear spectroscopy for studying and detecting recognition between biomolecules.

UI MeSH Term Description Entries
D008024 Ligands A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed) Ligand
D008239 Lysine An essential amino acid. It is often added to animal feed. Enisyl,L-Lysine,Lysine Acetate,Lysine Hydrochloride,Acetate, Lysine,L Lysine
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D002124 Calcium Fluoride Calcium fluoride. Occurring in nature as the mineral fluorite or fluorspar. It is the primary source of fluorine and its compounds. Pure calcium fluoride is used as a catalyst in dehydration and dehydrogenation and is used to fluoridate drinking water. (From Merck Index, 11th ed) Fluoride, Calcium
D001360 Avidin A specific protein in egg albumin that interacts with BIOTIN to render it unavailable to mammals, thereby producing biotin deficiency.
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D013055 Spectrophotometry, Infrared Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) IR Spectra,Infrared Spectrophotometry,IR Spectras,Spectra, IR
D013499 Surface Properties Characteristics or attributes of the outer boundaries of objects, including molecules. Properties, Surface,Property, Surface,Surface Property

Related Publications

Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Probing the extracellular matrix with sum-frequency-generation spectroscopy.
December 2008, Langmuir : the ACS journal of surfaces and colloids,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Probing Silica-Kaolinite Interactions with Sum Frequency Generation Spectroscopy.
December 2022, Langmuir : the ACS journal of surfaces and colloids,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Probing ligand removal and ordering at quantum dot surfaces using vibrational sum frequency generation spectroscopy.
March 2019, Journal of colloid and interface science,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Sample cells for probing solid/liquid interfaces with broadband sum-frequency-generation spectroscopy.
June 2010, The Review of scientific instruments,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Probing the spontaneous membrane insertion of a tail-anchored membrane protein by sum frequency generation spectroscopy.
November 2010, Journal of the American Chemical Society,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Probing Molecular Recognition at the Solid-Gas Interface by Sum-Frequency Vibrational Spectroscopy.
August 2016, The journal of physical chemistry letters,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Probing the electrode-solution interfaces in rechargeable batteries by sum-frequency generation spectroscopy.
November 2020, The Journal of chemical physics,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Probing of lipase activity at air/water interface by sum-frequency generation spectroscopy.
April 2008, The journal of physical chemistry. B,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Multireflection sum frequency generation vibrational spectroscopy.
August 2015, Analytical chemistry,
Laurent Dreesen, and Yannick Sartenaer, and Christophe Humbert, and Alaa A Mani, and Christophe Méthivier, and Claire-Marie Pradier, and Paul A Thiry, and André Peremans
Probing the orientation of surface-immobilized protein G B1 using ToF-SIMS, sum frequency generation, and NEXAFS spectroscopy.
November 2010, Langmuir : the ACS journal of surfaces and colloids,
Copied contents to your clipboard!