BIOMAT Database Logo BIOMAT Database Logo

Inhibition of osteopontin expression and function in oral cancer cell lines by antisense oligonucleotides. 2005

Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Department of Pathology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, Japan. tmuramat@tdc.ac.jp

We examined expression and function of osteopontin (OPN) in oral cancer cell lines using antisense oligonucleotide (AS). Quantitative real-time RT-PCR showed that expression in BSC-OF cells was significantly higher (10-fold) than that in KB cell. AS-study showed that foci of AS-treated BSC-OF cells possessed thin processes and radiated morphologically, although BSC-OF cells showed round foci. Cell growth in AS-group was lower (<80%) than the control. Invasion ability in AS-group became significantly lower (P<0.01). These results suggest that BSC-OF cell is useful for over-expression of OPN, and that OPN contributes to morphology, growth and invasion.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D009062 Mouth Neoplasms Tumors or cancer of the MOUTH. Cancer of Mouth,Mouth Cancer,Oral Cancer,Oral Neoplasms,Cancer of the Mouth,Neoplasms, Mouth,Neoplasms, Oral,Cancer, Mouth,Cancer, Oral,Cancers, Mouth,Cancers, Oral,Mouth Cancers,Mouth Neoplasm,Neoplasm, Mouth,Neoplasm, Oral,Oral Cancers,Oral Neoplasm
D009361 Neoplasm Invasiveness Ability of neoplasms to infiltrate and actively destroy surrounding tissue. Invasiveness, Neoplasm,Neoplasm Invasion,Invasion, Neoplasm
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012795 Sialoglycoproteins Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities. Polysialoglycoprotein,Sialoglycopeptide,Sialoglycopeptides,Sialoglycoprotein,Sialoprotein,Sialoproteins,Polysialoglycoproteins
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D016376 Oligonucleotides, Antisense Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize. Anti-Sense Oligonucleotide,Antisense Oligonucleotide,Antisense Oligonucleotides,Anti-Sense Oligonucleotides,Anti Sense Oligonucleotide,Anti Sense Oligonucleotides,Oligonucleotide, Anti-Sense,Oligonucleotide, Antisense,Oligonucleotides, Anti-Sense
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D053495 Osteopontin A negatively-charged extracellular matrix protein that plays a role in the regulation of BONE metabolism and a variety of other biological functions. Cell signaling by osteopontin may occur through a cell adhesion sequence that recognizes INTEGRIN ALPHA-V BETA-3. Bone Sialoprotein 1,Bone Sialoprotein I,Secreted Phosphoprotein 1,Sialoprotein 1,Uropontin,Sialoprotein 1, Bone,Sialoprotein I, Bone

Related Publications

Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Inhibition of TF gene expression by antisense oligonucleotides in different cancer cell lines.
December 2004, Journal of experimental therapeutics & oncology,
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Control of cyclin D1 expression by antisense oligonucleotides in three ovarian cancer cell lines.
September 1998, Gynecologic oncology,
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Growth inhibition of human tumor cell lines by antisense oligonucleotides designed to inhibit p120 expression.
February 1993, Anti-cancer drug design,
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Effects of antisense oligonucleotides targeting k-ras expression in pancreatic-cancer cell-lines.
May 1995, International journal of oncology,
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Inhibition of Rac function using antisense oligonucleotides.
January 1995, Methods in enzymology,
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Inhibition of endothelial cell adhesion molecule expression with antisense oligonucleotides.
April 1994, Journal of immunology (Baltimore, Md. : 1950),
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Specific inhibition of DNMT1 by antisense oligonucleotides induces re-expression of estrogen receptor-alpha (ER) in ER-negative human breast cancer cell lines.
January 2003, Cancer biology & therapy,
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
CD4 expression decrease by antisense oligonucleotides: inhibition of rat T CD4+ cell reactivity.
January 2003, Oligonucleotides,
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Expression of Pax-2 in human renal cell carcinoma and growth inhibition by antisense oligonucleotides.
September 1995, Cancer research,
Takashi Muramatsu, and Kaori Shima, and Kazumasa Ohta, and Harutoshi Kizaki, and Yasufumi Ro, and Yohko Kohno, and Yoshihiro Abiko, and Masaki Shimono
Inhibition of MDR1 gene expression by chimeric HNA antisense oligonucleotides.
January 2004, Nucleic acids research,
Copied contents to your clipboard!