Degradation of juvenile hormone and reproductive function during starvation and experimental increase of the juvenile hormone titer were studied in wild type and mutant D. virilis females incapable to respond to heat stress by changes in juvenile hormone metabolism and fertility. After 24-hour starvation, the females of both lines were characterized by a decreased level of juvenile hormone degradation, 24-hour delay of egg laying, increased egg laying within 3 h after the termination of starvation, and decreased fertility within three days. Application of exogenous juvenile hormone also led to a decreased level of its degradation and 24-hour arrest of egg laying. Experimental increase of the juvenile hormone titer before the beginning of starvation led to a sharply increased fertility (number of laid eggs and number of progenies) within the first 24 h after the termination of starvation. The dynamics of juvenile hormone degradation and of fertility were similar after starvation and upon application of the exogenous hormone. The role of juvenile hormone in the control of egg maturation and laying under stress conditions has been discussed.