Biomaterial Database

[Isolation of immunoglobulins by thiophilic adsorption chromatography]. 2004

F Marecek

The separation of antibodies is a process of great importance. The present paper deals with the method of thiophilic adsorption chromatography (TAC) based on the thiophilic sorbents. Thiophilic sorbents can interact with the immunoglobulins very selectively. Binding of immunoglobulins is dependent on the high ionic strength of the medium. Sorbed immunoglobulins are released by the ionic strength decay. TAC provides immunoglobulins from the plasma (or other sources) in one step. Purity of immunoglobulins is comparable with results of the other methods. Benefits of TAC are delicacy to immunoglobulins, simple handling and low costs of separation. By means of TAC it is possible to eliminate immunoglobulins from biological materials. Thiophilic sorbents are currently available but the possibilities of their exploitation are practically unknown.

UI	MeSH Term	Description	Entries
D007136	Immunoglobulins	Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.	Globulins, Immune,Immune Globulin,Immune Globulins,Immunoglobulin,Globulin, Immune
D002845	Chromatography	Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.	Chromatographies
D000327	Adsorption	The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.	Adsorptions