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Interlaboratory comparison of PCR-based identification and genogrouping of Neisseria meningitidis. 2005
Muhamed-Kheir Taha, and
Jean-Michel Alonso, and
Mary Cafferkey, and
Dominique A Caugant, and
Stuart C Clarke, and
Mathew A Diggle, and
Andrew Fox, and
Matthias Frosch, and
Stephen J Gray, and
Malcolm Guiver, and
Sigrid Heuberger, and
Jitka Kalmusova, and
Konstantinos Kesanopoulos, and
Anne-Marie Klem, and
Paula Kriz, and
John Marsh, and
Paula Mölling, and
Karen Murphy, and
Per Olcén, and
Oumar Sanou, and
Georgina Tzanakaki, and
Ulrich Vogel
Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France. mktaha@pasteur.fr
Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
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