Biomaterial Database

Glycosaminoglycan fractions in normal human urine. 1975

C A Pennock, and R G Charles, and D Stansbie

Department of Child Health, University of Bristol.

A study has been made of glycosaminoglycans in normal urine to determine which are non-dialysable, which are ultrafilterable, and which are precipitable with cetypyridinium chloride or alcohol. The main fractions present in human urine are: (1) High molecular weight material which is non-dialysable and precipitated by alcohol and by cetylpyridinium chloride. (2) High molecular weight material which is non-dialysable and precipitated by alcohol but not by cetylpyridinium chloride. (3) Low molecular weight material which is ultrafilterable and precipitated by alcohol and by cetylpyridinium chloride. (4) Low molecular weight material which is ultrafilterable and precipitated by alcohol but not by cetyipyridinium chloride. The heavy losses of glycosaminoglycans on dialysis have led us to conclude that this method should not be used to study the excretion of these polymers and that direct precipitation with cetylpyridinium chloride, which can be used to isolate both non-dialysable and ultrafilterable macromolecular fractions relevant to the diagnosis of the mucopolysaccharidoses, is the method of choice.

UI	MeSH Term	Description	Entries
D007223	Infant	A child between 1 and 23 months of age.	Infants
D008401	Gas Chromatography-Mass Spectrometry	A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.	Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D009083	Mucopolysaccharidoses	Group of lysosomal storage diseases each caused by an inherited deficiency of an enzyme involved in the degradation of glycosaminoglycans (mucopolysaccharides). The diseases are progressive and often display a wide spectrum of clinical severity within one enzyme deficiency.	Mucopolysaccharidosis
D011232	Chemical Precipitation	The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.	Precipitation, Chemical
D002594	Cetylpyridinium	Cationic bactericidal surfactant used as a topical antiseptic for skin, wounds, mucous membranes, instruments, etc.; and also as a component in mouthwash and lozenges.	Angifonil,Biosept,Catamium,Ceepryn Chloride,Cetamium,Cetylpyridinium Chloride,Cetylpyridinium Chloride Anhydrous,Cetylpyridium,Cetylyre,Dobendan,Hexadecylpyridinium,Merocets,Pristacin,Pyrisept,Sterogenol,Anhydrous, Cetylpyridinium Chloride,Chloride Anhydrous, Cetylpyridinium,Chloride, Ceepryn,Chloride, Cetylpyridinium
D002675	Child, Preschool	A child between the ages of 2 and 5.	Children, Preschool,Preschool Child,Preschool Children
D005374	Filtration	A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Filtrations
D006025	Glycosaminoglycans	Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine (see ACETYLGLUCOSAMINE) or N-acetylgalactosamine (see ACETYLGALACTOSAMINE).	Glycosaminoglycan,Mucopolysaccharides
D006595	Hexosamines	AMINO SUGARS created by adding an amine group to a hexose sugar.	Hexosamine