Biomaterial Database

Rapid determination of tramadol in human plasma by headspace solid-phase microextraction and capillary gas chromatography-mass spectrometry. 2005

Y F Sha, and S Shen, and G L Duan

Department of Pharmacy, Fudan University, Shanghai 200032, PR China.

A simple, rapid and sensitive method for determination of tramadol in plasma samples was developed using headspace solid-phase microextraction (HS-SPME) and gas chromatography with mass spectrometry (GC-MS). The optimum conditions for the SPME procedure were: headspace extraction on a 65-microm polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber; 0.5 mL of plasma modified with 0.5 mL of sodium hydroxide (0.1 M); extraction temperature of 100 degrees C, with stirring at 2000 rpm for 30 min. The calibration curve showed linearity in the range of 1-400 ng mL(-1) with regression coefficient corresponding to 0.9986 and coefficient of the variation of the points of the calibration curve lower than 10%. The detection limit for tramadol in plasma was 0.2 ng mL(-1). The proposed method was successfully applied to determination of tramadol in human plasma samples from 10 healthy volunteers after a single oral administration.

UI	MeSH Term	Description	Entries
D008401	Gas Chromatography-Mass Spectrometry	A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.	Chromatography, Gas-Liquid-Mass Spectrometry,Chromatography, Gas-Mass Spectrometry,GCMS,Spectrometry, Mass-Gas Chromatography,Spectrum Analysis, Mass-Gas Chromatography,Gas-Liquid Chromatography-Mass Spectrometry,Mass Spectrometry-Gas Chromatography,Chromatography, Gas Liquid Mass Spectrometry,Chromatography, Gas Mass Spectrometry,Chromatography, Mass Spectrometry-Gas,Chromatography-Mass Spectrometry, Gas,Chromatography-Mass Spectrometry, Gas-Liquid,Gas Chromatography Mass Spectrometry,Gas Liquid Chromatography Mass Spectrometry,Mass Spectrometry Gas Chromatography,Spectrometries, Mass-Gas Chromatography,Spectrometry, Gas Chromatography-Mass,Spectrometry, Gas-Liquid Chromatography-Mass,Spectrometry, Mass Gas Chromatography,Spectrometry-Gas Chromatography, Mass,Spectrum Analysis, Mass Gas Chromatography
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D014147	Tramadol	A narcotic analgesic proposed for severe pain. It may be habituating.	Adolonta,Amadol,Biodalgic,Biokanol,Contramal,Jutadol,K-315,MTW-Tramadol,Nobligan,Prontofort,Ranitidin 1A Pharma,Takadol,Theradol,Tiral,Topalgic,Tradol,Tradol-Puren,Tradonal,Tralgiol,Trama 1A Pharma,Trama AbZ,Trama KD,Trama-Dorsch,Tramabeta,Tramadin,Tramadoc,Tramadol 1A,Tramadol AL,Tramadol Asta Medica,Tramadol Basics,Tramadol Bayvit,Tramadol Bexal,Tramadol Cinfa,Tramadol Edigen,Tramadol Heumann,Tramadol Hydrochloride,Tramadol Kern,Tramadol Lichtenstein,Tramadol Lindo,Tramadol Mabo,Tramadol Normon,Tramadol PB,Tramadol Ratiopharm,Tramadol Stada,Tramadol acis,Tramadol-Dolgit,Tramadol-Hameln,Tramadol-ratiopharm,Tramadolor,Tramadura,Tramagetic,Tramagit,Tramake,Tramal,Tramex,Tramundin,Trasedal,Ultram,Xymel 50,Zamudol,Zumalgic,Zydol,Zytram,K 315,K315,MTW Tramadol,MTWTramadol,Tradol Puren,TradolPuren,Trama Dorsch,TramaDorsch,Tramadol Dolgit,Tramadol Hameln,TramadolDolgit,TramadolHameln,Tramadolratiopharm
D019075	Electrophoresis, Capillary	A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)	Capillary Zone Electrophoresis,Capillary Electrophoreses,Capillary Electrophoresis,Capillary Zone Electrophoreses,Electrophoreses, Capillary,Electrophoreses, Capillary Zone,Electrophoresis, Capillary Zone,Zone Electrophoreses, Capillary,Zone Electrophoresis, Capillary