Transcriptional regulation of FKLF-2 (KLF13) gene in erythroid cells. 2005

Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
Department of Hematology, Nagoya University Graduate School of Medicine, Nagoya, Japan.

FKLF-2 (KLF13) was cloned from fetal globin-expressing tissues and has been shown to be abundantly expressed in erythroid cells. In this study we examined the transcriptional regulation of the KLF13 gene. A 5.5 kb 5' flanking region cloned from mouse erythroleukemia (MEL) cell genomic DNA showed that major cis regulatory activities exist in the 550 bp sequence to the unique transcription start site, and that the promoter is more active in K562 cells than in COS-7 cells. The promoter was trans-activated by co-expressed GATA-1 through the sequence containing two CCAAT motifs, suggesting that GATA-1 is involved in the abundant expression of KLF13 mRNA in the erythroid tissue. Dual action, i.e. activating effect in COS-7 and repressive effect in K562 cell, was observed on its own promoter, suggesting a feedback mechanism for the transcriptional control of the KLF13 gene in the erythroid environment. These findings provide an insight on the mechanism of inducible mRNA expression of the KLF13 gene in erythroid cells.

UI MeSH Term Description Entries
D012097 Repressor Proteins Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release. Repressor Molecules,Transcriptional Silencing Factors,Proteins, Repressor,Silencing Factors, Transcriptional
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D015972 Gene Expression Regulation, Neoplastic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. Neoplastic Gene Expression Regulation,Regulation of Gene Expression, Neoplastic,Regulation, Gene Expression, Neoplastic
D051741 Kruppel-Like Transcription Factors A family of zinc finger transcription factors that share homology with Kruppel protein, Drosophila. They contain a highly conserved seven amino acid spacer sequence in between their ZINC FINGER MOTIFS. Kruppel-Like Factor,Kruppel-Like Transcription Factor,Kruppel-Like Factors,Factor, Kruppel-Like,Factor, Kruppel-Like Transcription,Kruppel Like Factor,Kruppel Like Factors,Kruppel Like Transcription Factor,Kruppel Like Transcription Factors,Transcription Factor, Kruppel-Like,Transcription Factors, Kruppel-Like

Related Publications

Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
February 2003, [Rinsho ketsueki] The Japanese journal of clinical hematology,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
June 2000, Blood,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
April 1979, Biochemistry,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
May 2001, The Biochemical journal,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
August 2004, The Journal of biological chemistry,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
July 1995, European journal of biochemistry,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
August 1996, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
September 1995, Nucleic acids research,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
April 2000, Gene,
Ayako Mitsuma, and Haruhiko Asano, and Tomohiro Kinoshita, and Takashi Murate, and Hidehiko Saito, and George Stamatoyannopoulos, and Tomoki Naoe
February 2000, The Journal of biological chemistry,
Copied contents to your clipboard!