Transforming growth factor-beta, 20-HETE interaction, and glomerular injury in Dahl salt-sensitive rats. 2005

Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
Department of Physiology, and Kidney Disease Center, Medical College of Wisconsin, Milwaukee, WI 53226-4801, USA.

This study examined the role of transforming growth factor-beta (TGF-beta) in altering the glomerular permeability to albumin (P(alb)) during hypertension development in Dahl salt-sensitive (Dahl S) rats and whether TGF-beta acts by inhibiting the glomerular production of 20-HETE. The results indicate that the renal expression of TGF-beta doubles in Dahl S rats fed a high-salt diet for 7 days, and this is associated with a marked rise in P(alb) from 0.19+/-0.04 to 0.75+/-0.01 and changes in the ultrastructure of the glomerular filtration barrier. Chronic treatment of Dahl S rats with a TGF-beta neutralizing antibody prevented the increase in P(alb) and preserved the structure of glomerular capillaries. It had no effect on the rise in blood pressure produced by the high-salt diet. In other studies, preincubation of glomeruli isolated from Sprague Dawley rats with TGF-beta1 (10 ng/mL) for 15 minutes increased P(alb) from 0.01+/-0.01 to 0.60+/-0.02. This was associated with inhibition of the glomerular production of 20-HETE from 221+/-11 to 3.4+/-0.5 mug per 30 minutes per milligram of protein. Pretreatment of Sprague Dawley glomeruli with a stable analog of 20-HETE, 20-hydroxyeicosa-5(Z), 14(Z)-dienoic acid, reduced baseline P(alb) and opposed the effects of TGF-beta to increase P(alb). These studies indicate that upregulation of the glomerular formation of TGF-beta may contribute to the development of proteinuria and glomerular injury early in hypertension development in Dahl S rats by increasing P(alb) through inhibition of the glomerular production of 20-HETE.

UI MeSH Term Description Entries
D006973 Hypertension Persistently high systemic arterial BLOOD PRESSURE. Based on multiple readings (BLOOD PRESSURE DETERMINATION), hypertension is currently defined as when SYSTOLIC PRESSURE is consistently greater than 140 mm Hg or when DIASTOLIC PRESSURE is consistently 90 mm Hg or more. Blood Pressure, High,Blood Pressures, High,High Blood Pressure,High Blood Pressures
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D007678 Kidney Glomerulus A cluster of convoluted capillaries beginning at each nephric tubule in the kidney and held together by connective tissue. Glomerulus, Kidney
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D010539 Permeability Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions. Permeabilities
D006893 Hydroxyeicosatetraenoic Acids Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes. HETE,Acids, Hydroxyeicosatetraenoic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012710 Serum Albumin, Bovine Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed) Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D016212 Transforming Growth Factor beta A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins. Bone-Derived Transforming Growth Factor,Platelet Transforming Growth Factor,TGF-beta,Milk Growth Factor,TGFbeta,Bone Derived Transforming Growth Factor,Factor, Milk Growth,Growth Factor, Milk
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats

Related Publications

Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
December 1996, Journal of the American Society of Nephrology : JASN,
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
March 2003, Hypertension (Dallas, Tex. : 1979),
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
January 1994, Experimental nephrology,
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
September 2014, American journal of physiology. Renal physiology,
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
November 1992, Hypertension (Dallas, Tex. : 1979),
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
July 1997, Current opinion in nephrology and hypertension,
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
November 2020, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
September 2014, American journal of physiology. Renal physiology,
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
October 2003, Hypertension (Dallas, Tex. : 1979),
Annette J Dahly-Vernon, and Mukut Sharma, and Ellen T McCarthy, and Virginia J Savin, and Steven R Ledbetter, and Richard J Roman
June 2000, Kidney international,
Copied contents to your clipboard!