BIOMAT Database Logo BIOMAT Database Logo

The gamma-actin encoding gene from the beta-carotene producer Blakeslea trispora. 2005

Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
R&D Biology, Antibióticos S.A., Avenida de Antibióticos 59-61, 24009 León, Spain.

We determined the nucleotide sequence of a 4599-bp DNA genomic fragment including the gamma-actin encoding gene from Blakeslea trispora, showing an open reading frame of 1561 bp interrupted by four introns with fungal consensus splice-site junctions. The untranslated regions of the actA gene contain a consensus TATA box, a CCAAT motif, a large pyrimidine stretch, and the polyadenylation sequence AATAAA. The predicted protein (375 amino acids) revealed high identity to gamma-actins from fungi (>90%), and gene phylogenies support the grouping of B. trispora actin close to those from the majority of the filamentous fungi. actA transcript (1.4 kb) level in beta-carotene producing conditions was faintly higher than carRA (1.9 kb) and slightly lower than carB (1.8 kb) beta-carotene biosynthetic genes. The use of the actA promoter (PactA) for heterologous gene expression was ascertained by the transformation of gene fusions with the bleomycin resistance gene (bleR) from Streptoalloteichus hindustanus and the geneticin resistance marker (aphI) from Tn903, into Escherichia coli and Acremonium chrysogenum.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004271 DNA, Fungal Deoxyribonucleic acid that makes up the genetic material of fungi. Fungal DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005656 Fungal Proteins Proteins found in any species of fungus. Fungal Gene Products,Fungal Gene Proteins,Fungal Peptides,Gene Products, Fungal,Yeast Proteins,Gene Proteins, Fungal,Peptides, Fungal,Proteins, Fungal
D005658 Fungi A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies. Fungi, Filamentous,Molds,Filamentous Fungi,Filamentous Fungus,Fungus,Fungus, Filamentous,Mold
D005800 Genes, Fungal The functional hereditary units of FUNGI. Fungal Genes,Fungal Gene,Gene, Fungal
D000199 Actins Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle. F-Actin,G-Actin,Actin,Isoactin,N-Actin,alpha-Actin,alpha-Isoactin,beta-Actin,gamma-Actin,F Actin,G Actin,N Actin,alpha Actin,alpha Isoactin,beta Actin,gamma Actin

Related Publications

Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
[Study of beta-carotene biosynthesis in Blakeslea trispora].
January 1967, Mikrobiologiia,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
An improved method for extraction of beta-carotene from Blakeslea trispora.
January 2001, Applied biochemistry and biotechnology,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
Blakeslea trispora genes for carotene biosynthesis.
September 2004, Applied and environmental microbiology,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
Effect of light on beta-carotene accumulation in Blakeslea trispora.
December 1970, Journal of general microbiology,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
Distribution of beta-carotene in subcellular fractions of Blakeslea trispora.
April 1970, Experientia,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
Activation of beta-carotene synthesis in Blakeslea trispora by certain terpenes.
January 1969, Acta chemica Scandinavica,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
Optimization of beta-carotene production from synthetic medium by Blakeslea trispora: a mathematical modeling.
May 2002, Applied biochemistry and biotechnology,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
Separation of beta-factor synthesis from stimulated beta-carotene synthesis in mated cultures of Blakeslea trispora.
February 1968, Journal of bacteriology,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
New insights into mechanisms of growth and beta-carotene production in Blakeslea trispora.
October 2006, Biotechnology journal,
Marta Rodríguez-Sáiz, and Ana Teresa Marcos, and Begoña Paz, and Juan Luis de la Fuente, and José Luis Barredo
Media optimization for the production of beta-carotene by Blakeslea trispora: a statistical approach.
March 2008, Bioresource technology,
Copied contents to your clipboard!