Thyroxine-binding antibodies inhibit T cell recognition of a pathogenic thyroglobulin epitope. 2005

Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
Faculty of Medicine, Memorial University of Newfoundland, St. John's, Newfoundland, Canada.

Thyroid hormone-binding (THB) Abs are frequently detected in autoimmune thyroid disorders but it is unknown whether they can exert immunoregulatory effects. We report that a THB mAb recognizing the 5' iodine atom of the outer phenolic ring of thyroxine (T4) can block T cell recognition of the pathogenic thyroglobulin (Tg) peptide (2549-2560) that contains T4 at aa position 2553 (T4(2553)). Following peptide binding to the MHC groove, the THB mAb inhibited activation of the A(k)-restricted, T4(2553)-specific, mouse T cell hybridoma clone 3.47, which does not recognize other T4-containing epitopes or noniodinated peptide analogues. Addition of the same THB mAb to T4(2553)-pulsed splenocytes largely inhibited specific activation of T4(2553)-primed lymph node cells and significantly reduced their capacity to adoptively transfer thyroiditis to naive CBA/J mice. These data demonstrate that some THB Abs can block recognition of iodine-containing Tg epitopes by autoaggressive T cells and support the view that such Abs may influence the development or maintenance of thyroid disease.

UI MeSH Term Description Entries
D007455 Iodine A nonmetallic element of the halogen group that is represented by the atomic symbol I, atomic number 53, and atomic weight of 126.90. It is a nutritionally essential element, especially important in thyroid hormone synthesis. In solution, it has anti-infective properties and is used topically. Iodine-127,Iodine 127
D008198 Lymph Nodes They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system. Lymph Node,Node, Lymph,Nodes, Lymph
D008213 Lymphocyte Activation Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION. Blast Transformation,Blastogenesis,Lymphoblast Transformation,Lymphocyte Stimulation,Lymphocyte Transformation,Transformation, Blast,Transformation, Lymphoblast,Transformation, Lymphocyte,Activation, Lymphocyte,Stimulation, Lymphocyte
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008808 Mice, Inbred CBA An inbred strain of mouse that is widely used in BIOMEDICAL RESEARCH. Mice, CBA,Mouse, CBA,Mouse, Inbred CBA,CBA Mice,CBA Mice, Inbred,CBA Mouse,CBA Mouse, Inbred,Inbred CBA Mice,Inbred CBA Mouse
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D011948 Receptors, Antigen, T-Cell Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (CD3 COMPLEX). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains. Antigen Receptors, T-Cell,T-Cell Receptors,Receptors, T-Cell Antigen,T-Cell Antigen Receptor,T-Cell Receptor,Antigen Receptor, T-Cell,Antigen Receptors, T Cell,Receptor, T-Cell,Receptor, T-Cell Antigen,Receptors, T Cell Antigen,Receptors, T-Cell,T Cell Antigen Receptor,T Cell Receptor,T Cell Receptors,T-Cell Antigen Receptors
D002999 Clone Cells A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed) Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D005260 Female Females
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma

Related Publications

Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
July 2007, Immunology,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
August 1991, The Journal of experimental medicine,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
August 2010, Journal of autoimmunity,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
August 1999, European journal of immunology,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
April 1996, European journal of immunology,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
July 1994, European journal of immunology,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
March 1995, The Journal of clinical endocrinology and metabolism,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
July 2013, Oncotarget,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
December 2012, Journal of cell science,
Yang D Dai, and Petros Eliades, and Karen A Carayanniotis, and Daniel J McCormick, and Yi-Chi M Kong, and Vassiliki Magafa, and Paul Cordopatis, and Peggy Lymberi, and George Carayanniotis
August 2003, Clinical endocrinology,
Copied contents to your clipboard!