BIOMAT Database Logo BIOMAT Database Logo

Isolation and characterization of a novel membrane glycoprotein of 85,000 molecular weight from rat liver lysosomes. 1992

K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.

We have purified and characterized a novel glycoprotein (r-lamp-3) with an apparent molecular weight (Mr) of 85,000 from membranes of triton-filled lysosomes (tritosomes) by the use of immunoaffinity chromatography on a column of monoclonal antibody-Sepharose 4B. r-lamp-3 accounted for approximately 4% of the total proteins in tritosomal membranes. The isoelectric point (pI) of r-lamp-3 was 4.5 and it was shifted to 6.5 after neuraminidase treatment with its molecular weight decreased by about 7000. Pulse-chase experiments in cultured rat hepatocytes using [35S]methionine showed that r-lamp-3 was initially synthesized as a 77,000 polypeptide and processed to a mature protein with an Mr of 85,000. Upon treatment with endo-beta-N-acetylglucosaminidase H (Endo H), the precursor and mature forms were converted to 55,000 and 73,000 polypeptides, respectively. From the Mr reduction of the precursor form, we estimated the presence of 10--12 N-linked oligosaccharides/r-lamp-3 polypeptide. The data on enzymatic deglycosylation suggested that the mature form of r-lamp-3 contained the same numbers of high mannose-type and complex-type N-linked oligosaccharide chains.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008247 Lysosomes A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured or undergoes MEMBRANE FUSION. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed). Autolysosome,Autolysosomes,Lysosome
D008297 Male Males
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Isolation and characterization of a high molecular weight glycoprotein from human blood platelets.
December 1978, The Journal of biological chemistry,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Isolation, purification, characterization and metabolism of high-molecular-weight folate from rat liver [proceedings].
January 1977, Biochemical Society transactions,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
[Intracellular protein degradation. IV. Isolation and characterization of peptidases from rat liver lysosomes].
January 1972, Acta biologica et medica Germanica,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Isolation and characterization of a membrane-bound proteinase from rat liver.
December 1976, Archives of biochemistry and biophysics,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Isolation and characterization of glycopeptides from rat liver nuclear membrane.
December 1972, Journal of biochemistry,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Isolation of a polydisperse high-molecular-weight glycogen from rat liver.
February 1981, Analytical biochemistry,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Isolation and characterization of a high-molecular-weight glycoprotein from the endometrium of porcine uteri.
December 1985, Experientia,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Isolation and characterization of chicken liver lysosomes.
June 1988, Biochemistry international,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
The isolation and characterization of the high-molecular-weight glycoprotein from pig colonic mucus.
August 1978, The Biochemical journal,
K Akasaki, and H Kinoshita, and M Fukuzawa, and M Maeda, and Y Yamaguchi, and K Furuno, and H Tsuji
Isolation and characterization of rat liver nuclear matrices containing high molecular weight deoxyribonucleic acid.
August 1981, Biochemistry,
Copied contents to your clipboard!