Biomaterial Database

Characterisation of T cell antigen receptor alpha chain isotypes in the common carp. 2005

Etsuou Imai, and Jun Ishikawa, and Tadaaki Moritomo, and Mitsuru Tomana

Department of Applied Biological Science, Nihon University, Fujisawa, Kanagawa, Japan.

T cell receptor alpha (TCRalpha) chain has been characterised in several teleost species to date. Here, a reverse transcription-polymerase chain reaction (RT-PCR) strategy was used to isolate cDNA clones encoding TCRalpha chain from an individual of the common carp (Cyprinus carpio.L.). The Valpha sequences identified were most similar to Valpha of other teleosts, and could be classified into as many as 14 Valpha families. For the Jalpha sequences, diversity comparable to that seen in other teleosts could be identified, and the J-region motif was well conserved. The Calpha sequences demonstrated the highest similarity to zebrafish Calpha and possessed a well-conserved transmembrane (TM) region. Two Calpha isotypes with a complete C region were obtained, designated Calpha1 and Calpha2, with approximately 70% similarity at the amino acid level ( approximately 85% identity at the nucleotide level), and, in addition, Calpha2 contained two unique sequences, designated Calpha2a and Calpha2b, with 93% similarity (96% identity). Therefore, the results obtained using an individual clearly showed that carp possesses at least two Calpha loci, possibly as a result of tetraploidisation.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002347	Carps	Common name for a number of different species of fish in the family Cyprinidae. This includes, among others, the common carp, crucian carp, grass carp, and silver carp.	Carassius carassius,Crucian Carp,Cyprinus,Grass Carp,Carp,Ctenopharyngodon idellus,Cyprinus carpio,Hypophthalmichthys molitrix,Koi Carp,Silver Carp,Carp, Crucian,Carp, Grass,Carp, Koi,Carp, Silver,Carps, Crucian,Carps, Grass,Carps, Silver,Crucian Carps,Grass Carps,Silver Carps
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014644	Genetic Variation	Genotypic differences observed among individuals in a population.	Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic
D015139	Blotting, Southern	A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.	Southern Blotting,Blot, Southern,Southern Blot
D015152	Blotting, Northern	Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.	Northern Blotting,Blot, Northern,Northern Blot,Blots, Northern,Blottings, Northern,Northern Blots,Northern Blottings
D015332	Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor	Ordered rearrangement of T-cell variable gene regions coding for the alpha-chain of antigen receptors.	T-Cell Antigen Receptor alpha-Chain Gene Rearrangement,T-Lymphocyte Antigen Receptor alpha-Chain Gene Rearrangement,Gene Rearrangement, alpha-Chain T Cell Antigen Receptor,T Cell alpha-Chain Gene Rearrangement,T Lymphocyte alpha-Chain Gene Rearrangement,Gene Rearrangement, alpha Chain T Cell Antigen Receptor,T Cell Antigen Receptor alpha Chain Gene Rearrangement,T Cell alpha Chain Gene Rearrangement,T Lymphocyte Antigen Receptor alpha Chain Gene Rearrangement,T Lymphocyte alpha Chain Gene Rearrangement
D016415	Sequence Alignment	The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.	Sequence Homology Determination,Determination, Sequence Homology,Alignment, Sequence,Alignments, Sequence,Determinations, Sequence Homology,Sequence Alignments,Sequence Homology Determinations