Improvement of doxazosin determination in human plasma using high-performance liquid chromatography with fluorescence detection. 2005

Pattana Sripalakit, and Penporn Nermhom, and Aurasorn Saraphanchotiwitthaya
Department of Pharmaceutical Chemistry and Pharmacognosy, Naresuan University, Phitsanulok 65000, Thailand. pattana9@excite.com

A simple, sensitive, rapid, and reproducible high-performance liquid chromatographic method is developed and validated for the determination of doxazosin in human plasma without a solvent extraction procedure. This method involves plasma protein precipitation using methanol. The structurally related compound prazosin is used as an internal standard. Doxazosin is detected with high sensitivity using spectrofluorimetry. Over the concentration range 0.5-20 ng/mL, the absolute recovery values are all greater than 98%. The method has a quantitation limit of 0.5 ng/mL. The intra- and interday coefficient of variation and inaccuracy values are all less than 8% and 7%, respectively. Therefore, the method has been applied in pharmacokinetic studies of doxazosin.

UI MeSH Term Description Entries
D011232 Chemical Precipitation The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution. Precipitation, Chemical
D001798 Blood Proteins Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins. Blood Protein,Plasma Protein,Plasma Proteins,Serum Protein,Serum Proteins,Protein, Blood,Protein, Plasma,Protein, Serum,Proteins, Blood,Proteins, Plasma,Proteins, Serum
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000432 Methanol A colorless, flammable liquid used in the manufacture of FORMALDEHYDE and ACETIC ACID, in chemical synthesis, antifreeze, and as a solvent. Ingestion of methanol is toxic and may cause blindness. Alcohol, Methyl,Carbinol,Sodium Methoxide,Wood Alcohol,Alcohol, Wood,Methoxide, Sodium,Methyl Alcohol
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D017292 Doxazosin A prazosin-related compound that is a selective alpha-1-adrenergic blocker. Doxazosin Mesylate,1 (4-Amino-6,7-dimethoxy-2-quinazolinyl)-4-((2,3-dihydro-1,4-benzodioxin-2-yl)carbonyl)piperazine,Alfamedin,Apo-Doxazosin,Cardular,Cardura,Carduran,Carduran Neo,Diblocin,Doxa-Puren,DoxaUro,Doxacor,Doxagamma,Doxamax,Doxatensa,Doxazomerck,Doxazosin AL,Doxazosin AZU,Doxazosin Apogepha,Doxazosin Heumann,Doxazosin Klast,Doxazosin Monohydrochloride,Doxazosin Stada,Doxazosin Von Ct,Doxazosin beta,Doxazosin findusFit,Doxazosin-Wolff,Doxazosin-ratiopharm,Doxazosina Alter,Doxazosina Cinfa,Doxazosina Combino Pharm,Doxazosina Geminis,Doxazosina Normon,Doxazosina Pharmagenus,Doxazosina Ratiopharm,Doxazosina Ur,Gen-Doxazosin,Jutalar,MTW-Doxazosin,Novo-Doxazosin,Progandol Neo,UK-33274,Uriduct,Zoxan,ratio-Doxazosin,Apo Doxazosin,Ct, Doxazosin Von,Doxa Puren,Doxazosin Wolff,Doxazosin ratiopharm,Gen Doxazosin,MTW Doxazosin,Mesylate, Doxazosin,Monohydrochloride, Doxazosin,Neo, Carduran,Novo Doxazosin,Ratiopharm, Doxazosina,UK 33274,UK33274,Von Ct, Doxazosin,ratio Doxazosin

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