Biomaterial Database

[Distribution of inducible nitric oxide synthase in different stages of Schistosoma japonicum]. 2004

Xiao-chun Long, and Wen-qi Liu, and Yong-long Li, and Andreas Rupple

Department of Parasitology, Tongji Medical College, Hua Zhong University of Science and Technology, Wuhan 430030, China.

OBJECTIVE To study the presence of iNOS in Schistosoma japonicum and demonstrate its distribution in different stages of this schistosome. METHODS Cryostat sections for adult worms and paraffin sections for eggs in the liver of infected mouse, sporocysts and cercariae in snails were prepared, immunofluorescent test was performed to detect the presence of iNOS in adult worms, sporocysts, cercariae and miracidium, the distribution of this enzyme was observed in different stages of Schistosoma japonicum. Western blotting was used to further demonstrate the immunoreactivity to iNOS in adult worms. RESULTS The results of immunofluorescent test showed that specific yellow-green fluorescence was mainly among subtugment of adult worms. Positive staining was also distributed on the surface of miracidium and its glands. For both sporocysts and cercariae, the majority of fluorescence was on their surface. Anti-iNOS antibody could recognize an apparent specific band in Western blotting of adult worm proteins, with a of Mr 210,000. CONCLUSIONS There is an expression of iNOS-like enzyme in Schistosoma japonicum.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012549	Schistosoma japonicum	A species of trematode blood flukes belonging to the family Schistosomatidae whose distribution is confined to areas of the ASIA, EASTERN. The intermediate host is a snail. It occurs in man and other mammals.	Schistosoma japonicums,japonicum, Schistosoma
D012908	Snails	Marine, freshwater, or terrestrial mollusks of the class Gastropoda. Most have an enclosing spiral shell, and several genera harbor parasites pathogenic to man.	Snail
D015153	Blotting, Western	Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.	Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D015200	Fluoroimmunoassay	The use of fluorescence spectrometry to obtain quantitative results for the FLUORESCENT ANTIBODY TECHNIQUE. One advantage over the other methods (e.g., radioimmunoassay) is its extreme sensitivity, with a detection limit on the order of tenths of microgram/liter.	Immunofluorometric Assay,Time-Resolved Immunofluorometric Assay,Assay, Immunofluorometric,Assay, Time-Resolved Immunofluorometric,Assays, Immunofluorometric,Assays, Time-Resolved Immunofluorometric,Fluoroimmunoassays,Immunofluorometric Assay, Time-Resolved,Immunofluorometric Assays,Immunofluorometric Assays, Time-Resolved,Time Resolved Immunofluorometric Assay,Time-Resolved Immunofluorometric Assays
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D052247	Nitric Oxide Synthase Type II	A CALCIUM-independent subtype of nitric oxide synthase that may play a role in immune function. It is an inducible enzyme whose expression is transcriptionally regulated by a variety of CYTOKINES.	INOS Enzyme,Inducible NOS Protein,Inducible Nitric Oxide Synthase,NOS-II,Nitric Oxide Synthase II,Nitric Oxide Synthase, Type II,NOS II