BIOMAT Database Logo BIOMAT Database Logo

Treponema medium glycoconjugate inhibits activation of human gingival fibroblasts stimulated with phenol-water extracts of periodontopathic bacteria. 2005

Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
Department of Oral Microbiology, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan.

Oral treponemes are well-known as causative agents of periodontal diseases; however, the details have not been fully clarified. Here, we examined the effects of Treponema medium glycoconjugate on the activation of human gingival fibroblasts using phenol-water extracts from Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum subsp. nucleatum, and Actinobacillus actinomycetemcomitans. The phenol-water extracts activated human gingival fibroblasts to mediate IL-8 production, as well as IL-8 mRNA expression, phosphorylation of p38 mitogen-activated protein kinase, and expression of intercellular adhesion molecule-1. T. medium glycoconjugate exhibited no activation of human gingival fibroblasts, while phenol-water extract-induced activation of human gingival fibroblasts was clearly inhibited by T. medium glycoconjugate. Furthermore, binding of biotinylated phenol-water extracts to CD14 in the presence of LPS-binding protein was blocked with T. medium glycoconjugate. These results suggest that T. medium glycoconjugate has an inhibitory effect on host cell activation by periodontopathic bacteria caused by binding to CD14- and LPS-binding protein.

UI MeSH Term Description Entries
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D010510 Periodontal Diseases Pathological processes involving the PERIODONTIUM including the gum (GINGIVA), the alveolar bone (ALVEOLAR PROCESS), the DENTAL CEMENTUM, and the PERIODONTAL LIGAMENT. Parodontosis,Pyorrhea Alveolaris,Disease, Periodontal,Diseases, Periodontal,Parodontoses,Periodontal Disease
D010636 Phenols Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002457 Cell Extracts Preparations of cell constituents or subcellular materials, isolates, or substances. Cell Extract,Extract, Cell,Extracts, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D005881 Gingiva Oral tissue surrounding and attached to TEETH. Gums,Interdental Papilla,Papilla, Interdental,Gum

Related Publications

Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
The effects of extracts from periodontopathic bacteria on human periodontal fibroblasts stimulated with mineralization supplements.
September 2003, Journal of oral science,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
Effect of human leukocyte extracts and gingival exudate on periodontopathic bacteria.
June 1983, Inflammation,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
[Effects of endotoxins from periodontopathic bacteria on the collagen metabolism of cultured normal human gingival fibroblasts].
September 1989, Nihon Shishubyo Gakkai kaishi,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
Treponema denticola outer membrane inhibits calcium flux in gingival fibroblasts.
February 1998, Infection and immunity,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.
September 2000, Infection and immunity,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
Triclosan inhibits tumor necrosis factor-alpha-stimulated urokinase production in human gingival fibroblasts.
December 2009, Journal of periodontal research,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
Black tea polyphenol inhibits CXCL10 production in oncostatin M-stimulated human gingival fibroblasts.
June 2011, International immunopharmacology,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
IL-10 inhibits Porphyromonas gingivalis LPS-stimulated human gingival fibroblasts production of IL-6.
September 1999, Biochemical and biophysical research communications,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
Ultrastructural studies of the effect of human leukocyte extracts on periodontopathic bacteria.
June 1983, Inflammation,
Y Asai, and Y Ohyama, and Y Taiji, and Y Makimura, and R Tamai, and M Hashimoto, and T Ogawa
Treponema denticola induces actin rearrangement and detachment of human gingival fibroblasts.
August 1992, Infection and immunity,
Copied contents to your clipboard!