Identification of Fritillaria pallidiflora using diagnostic PCR and PCR-RFLP based on nuclear ribosomal DNA internal transcribed spacer sequences. 2005

Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
Key Laboratory of Modern Traditional Chinese Medicines, and Department of Pharmacognosy, School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, Jiangsu Province, PR China.

Fritillaria pallidiflora Schrenk (Liliaceae) is a commonly used antitussive herb. There are 9 species of Fritillaria recorded as herbal drugs in the Chinese Pharmacopoeia. The other species are often marketed as F. pallidiflora, and thus, the therapeutic effects of F. pallidiflora are not achieved. Methods to distinguish F. pallidiflora from the 8 other species of Fritillaria are limited by the current morphological and chemical methods. In this study, we report two molecular authentication methods based on the sequences of nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) regions. For diagnostic PCR, we designed a pair of species-specific primers to authenticate F. pallidiflora. The PCR program consisted of only two steps for every repeated cycle. For PCR-RFLP, we identified a distinctive site which can be recognized by the restriction endonuclease Eco81I in the nrDNA ITS1 region of F. pallidiflora. PCR-RFLP analysis was established to differentiate F. pallidiflora from the other species of Fritillaria. These methods provide effective and accurate identification of F. pallidiflora.

UI MeSH Term Description Entries
D008517 Phytotherapy Use of plants or herbs to treat diseases or to alleviate pain. Herb Therapy,Herbal Therapy
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D017931 DNA Primers Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques. DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide
D018744 DNA, Plant Deoxyribonucleic acid that makes up the genetic material of plants. Plant DNA
D021903 DNA, Ribosomal Spacer The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers). Ribosomal Spacer DNA,Spacer DNA, Ribosomal
D031410 Fritillaria A plant genus of the family LILIACEAE. Members of this genus produce imperialine, a steroidal alkaloid which acts at muscarinic receptors. Fritillary,Fritillarias,Fritillaries

Related Publications

Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
January 1999, American journal of botany,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
February 1999, Molecular phylogenetics and evolution,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
June 2006, Journal of dermatological science,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
January 2001, Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
November 2006, Nan fang yi ke da xue xue bao = Journal of Southern Medical University,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
December 2005, Biochemical genetics,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
March 1998, TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
January 2001, Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
April 1999, Journal of clinical microbiology,
Chong-Zhi Wang, and Ping Li, and Jia-Yi Ding, and Guo-Qian Jin, and Chun-Su Yuan
April 1999, American journal of botany,
Copied contents to your clipboard!