Cooling and freezing of boar spermatozoa: supplementation of the freezing media with reduced glutathione preserves sperm function. 2005

Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
Department of Physiology, Facultad de Veterinaria, Universidad de Murcia, 30100 Murcia, Spain. jgadea@um.es

In this study, we evaluated the effects of glutathione (L-gamma-glutamyl-L-cysteinylglycine; GSH) supplementation of the freezing extender on semen parameters during the cooling (2 hours at 5 degrees C) and freezing phases of the cryopreservation process to compensate for the decrease in GSH content observed during sperm freezing. To fully address these questions, we incorporated a new set of functional sperm tests. These included tests of mitochondrial function, inducibility of the acrosome reaction, in vitro penetration (IVP) of oocytes, changes in sulfhydryl group content in membrane proteins, and capacitation status. The main findings emerging from this study were that the addition of GSH to the freezing media resulted in 1) an improvement in percent motility (%MOT) and motion parameters of thawed spermatozoa, as measured by both microscopic analysis and computer-assisted semen analysis (CASA); 2) a higher number of total viable spermatozoa; 3) a higher number of noncapacitated viable spermatozoa; and 4) a decrease in the number of spermatozoa with changes in the sulfhydryl groups in membrane proteins. This protective effect on sperm function was more pronounced with 1 mM of GSH than with 5 mM of GSH.

UI MeSH Term Description Entries
D007425 Intracellular Membranes Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES. Membranes, Intracellular,Intracellular Membrane,Membrane, Intracellular
D008297 Male Males
D008564 Membrane Potentials The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization). Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D003451 Cryoprotective Agents Substances that provide protection against the harmful effects of freezing temperatures. Cryoprotective Agent,Cryoprotective Effect,Cryoprotective Effects,Agent, Cryoprotective,Agents, Cryoprotective,Effect, Cryoprotective,Effects, Cryoprotective
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005615 Freezing Liquids transforming into solids by the removal of heat. Melting
D005978 Glutathione A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides. Reduced Glutathione,gamma-L-Glu-L-Cys-Gly,gamma-L-Glutamyl-L-Cysteinylglycine,Glutathione, Reduced,gamma L Glu L Cys Gly,gamma L Glutamyl L Cysteinylglycine
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012662 Semen Preservation The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Frozen Semen,Sperm Preservation,Preservation, Semen,Preservation, Sperm,Semen, Frozen
D013081 Sperm Motility Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression. Motilities, Sperm,Motility, Sperm,Sperm Motilities

Related Publications

Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
January 2005, Journal of andrology,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
January 2006, Cryo letters,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
December 2017, Cryobiology,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
September 2018, Animal reproduction science,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
August 2004, Theriogenology,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
January 2015, Polish journal of veterinary sciences,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
March 2013, Reproductive biology,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
April 2022, Theriogenology,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
February 1994, Zentralblatt fur Veterinarmedizin. Reihe A,
Joaquín Gadea, and Francisco García-Vazquez, and Carmen Matás, and Juan C Gardón, and Sebastián Cánovas, and David Gumbao
October 2020, Cryobiology,
Copied contents to your clipboard!