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We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The duplex-specific nuclease is thermostable, it effectively cleaves double-stranded DNA and is inactive toward single-stranded DNA (Shagin et al., Genome Res., 2002, vol. 12, pp. 1935-1942). Our method enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation. The efficiency of the method was demonstrated in model experiments using cDNA samples from several human tissues.
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
May 2003,
BioTechniques,
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
January 2001,
Methods in molecular biology (Clifton, N.J.),
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
January 2003,
Methods in molecular biology (Clifton, N.J.),
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
September 2008,
Analytical biochemistry,
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
October 2012,
International journal of molecular sciences,
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
January 2009,
Methods in molecular biology (Clifton, N.J.),
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
January 2012,
Methods in molecular biology (Clifton, N.J.),
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
January 2002,
In silico biology,
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
March 2001,
BioTechniques,
P A Zhulidov, and
E A Bogdanova, and
A S Shcheglov, and
I A Shagina, and
L L Vagner, and
G L Khazpekov, and
V V Kozhemiako, and
S A Luk'ianov, and
D A Shagin
January 1989,
Gene analysis techniques,
