Biomaterial Database

Immunogenic cross-reaction among outer membrane proteins of Gram-negative bacteria. 2005

Changxin Xu, and Sanying Wang, and Zhang Zhaoxia, and Xuanxian Peng

Department of Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, P.R. China.

In the present study, antigenic cross-reactivity of OMPs was investigated in several species of bacterial pathogens. Heterogeneous mouse or fish antisera were used to ascertain OMPs with cross-reactivity and cluster analysis was performed to analyze the distribution of cross-antigenic OMPs in diverse bacterial strains. We interestingly found that eleven and seven bands could be reacted with four kinds of heterogeneous mouse and fish antisera, respectively, and the phenograms constructed could provide ideal targeted bacteria for candidate genes of polyvalent vaccines. Importantly, there were significant differences in reaction with bacteria between mouse and fish antisera, but commonly antigenic bands still existed between them. Our results suggest that the cross-reactivity of OMPs exists commonly in Gram-negative bacteria, which may be a promising choice for the development of polyvalent OMP vaccines. Meanwhile, cluster analysis will help to understand the relation of cross-antigenic OMPs among Gram-negative bacteria.

UI	MeSH Term	Description	Entries
D002347	Carps	Common name for a number of different species of fish in the family Cyprinidae. This includes, among others, the common carp, crucian carp, grass carp, and silver carp.	Carassius carassius,Crucian Carp,Cyprinus,Grass Carp,Carp,Ctenopharyngodon idellus,Cyprinus carpio,Hypophthalmichthys molitrix,Koi Carp,Silver Carp,Carp, Crucian,Carp, Grass,Carp, Koi,Carp, Silver,Carps, Crucian,Carps, Grass,Carps, Silver,Crucian Carps,Grass Carps,Silver Carps
D003429	Cross Reactions	Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.	Cross Reaction,Reaction, Cross,Reactions, Cross
D006090	Gram-Negative Bacteria	Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.	Gram Negative Bacteria
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001425	Bacterial Outer Membrane Proteins	Proteins isolated from the outer membrane of Gram-negative bacteria.	OMP Proteins,Outer Membrane Proteins, Bacterial,Outer Membrane Lipoproteins, Bacterial
D001428	Bacterial Vaccines	Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.	Bacterial Vaccine,Bacterin,Vaccine, Bacterial,Vaccines, Bacterial
D016000	Cluster Analysis	A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.	Clustering,Analyses, Cluster,Analysis, Cluster,Cluster Analyses,Clusterings