Purification and partial characterization of an extracellular ribonuclease from a mutant of Aspergillus niger. 2005

Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
Biotechnology Research Center and The Key Laboratory of Gene Engineering of Ministry of Education, Zhongshan University, Guangzhou 510275, P.R.China.

A new extracellular ribonuclease (RNase) from a mutant of Aspergillus niger, named A. niger SA-13-20 RNase, was purified to homogeneity by (NH4)2SO4 fractionation (50-85%), DEAE-cellulose anion-exchange chromatography, ultrafiltration and Sephacryl HR-200 chromatography. The enzyme was purified up to 54.4-fold with a final yield of 24.5%. There were differences in the molecular weight, pI value and some physico-chemical properties between A. niger SA-13-20 RNase and that from the parent strain. The enzyme is monomeric and its molecular weight and isoelectric point were 40.1 kDa and 5.3, respectively. The N-terminal amino acid sequence of A. niger SA-13-20 RNase was TIDTYSSDSP. The optimum pH, temperature and buffer concentration for the enzymatic reaction were 3.5, 65 degrees C, and 0.175 M, respectively. Metal ions, such as K+, NH4+, Mg2+, and Ca2+ at the concentration of 1.0 mM had a slight activation effect on the enzyme activity and (NH4)2SO4 activated the enzyme significantly. The enzyme was stable at pH lower than 8.5 and was easy to inactivate in strong alkali solution.

UI MeSH Term Description Entries
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D004789 Enzyme Activation Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme. Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D004795 Enzyme Stability The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat. Enzyme Stabilities,Stabilities, Enzyme,Stability, Enzyme
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D001234 Aspergillus niger An imperfect fungus causing smut or black mold of several fruits and vegetables such as grapes, apricots, onions, and peanuts, and is a common contaminant of food. Aspergillus lacticoffeatus
D012260 Ribonucleases Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-. Nucleases, RNA,RNase,Acid Ribonuclease,Alkaline Ribonuclease,Ribonuclease,RNA Nucleases,Ribonuclease, Acid,Ribonuclease, Alkaline
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures

Related Publications

Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
July 2015, Journal of food science and technology,
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
January 2003, Bioresource technology,
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
January 1980, The International journal of biochemistry,
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
January 1998, Acta microbiologica Polonica,
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
February 1976, Biochimica et biophysica acta,
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
July 2000, Journal of biotechnology,
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
March 2009, Indian journal of microbiology,
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
October 2009, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology],
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
January 1994, Applied biochemistry and biotechnology,
Ya-Hong Xiong, and Jian-Zhong Liu, and Hai-Yan Song
December 1997, FEMS microbiology letters,
Copied contents to your clipboard!