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OBJECTIVE To assess the effect of crystalline triamcinolone acetonide on retinal endothelial cell proliferation in vivo and in vitro. METHODS For in vitro analysis, a sprouting assay was employed. Bovine retinal endothelial cells were stimulated with basic fibroblast growth factor (bFGF) and incubated with different concentrations of triamcinolone acetonide (0.05 mg/ml to 8 mg/ml). For in vivo analysis, a retinopathy of prematurity (ROP) model was used. 16 C57BL/J6 mice were exposed to 75% oxygen from postnatal day 7 to day 12. On day 12, triamcinolone acetonide was intravitreally injected into one eye ("study eye") and isotonic saline into the contralateral eye ("control eye"). On day 17, the mice were sacrificed and the eyes removed for quantitative analysis of preretinal neovascularisation. Four non-exposed mice served as negative control. RESULTS The sprouting assay demonstrated a dose dependent inhibition of bovine retinal endothelial cell proliferation from 0.05 mg triamcinolone acetonide/ml (no inhibition) to 3 mg triamcinolone acetonide/ml (complete inhibition). Dosages of more than 2 mg/ml resulted in cytotoxic changes of endothelial cells. The ROP model demonstrated a significantly lower neovascular cell count of 58% in the study group compared to the control group (6.35 (SD 2.1) cells per histological section versus 14.9 (SD 5.3) cells; p<0.005). CONCLUSIONS Triamcinolone acetonide inhibits bFGF induced proliferation of retinal endothelial cells in vivo and in vitro. These findings contribute to understanding the mode of action and effects of triamcinolone acetonide on retinal neovascularisation.
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
November 2007,
Acta ophthalmologica Scandinavica,
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
July 2008,
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
January 2011,
European journal of ophthalmology,
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
June 2007,
Investigative ophthalmology & visual science,
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
May 2009,
American journal of ophthalmology,
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
February 2006,
Investigative ophthalmology & visual science,
U H M Spandau, and
G Sauder, and
U Schubert, and
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J B Jonas
January 2017,
Current neurovascular research,
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
January 2005,
European journal of ophthalmology,
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
January 2013,
Retina (Philadelphia, Pa.),
U H M Spandau, and
G Sauder, and
U Schubert, and
H-P Hammes, and
J B Jonas
September 2006,
Journal of cataract and refractive surgery,
