OBJECTIVE To investigate the in vitro enamel and dentine wear by toothpastes with a range of Relative Dentine Abrasivity (RDA) and Relative Enamel Abrasivity (REA) values. METHODS Human enamel/dentine (approximately 50:50) blocks (approximately 4 x 4 mm) were polished and the enamel indented with four Knoop indents. The step across the enamel-dentine junction was measured by interference microscopy. Specimens were brushed in vitro with toothpastes with a range of RDA/REA values using a flat trim toothbrush mounted in a Wira brushing machine for 360 s and a load of 375 g. After brushing the Knoop indent length and the enamel-dentine step were remeasured and the amount of wear to the enamel and dentine was calculated. RESULTS The mean enamel wear ranged from 0.05 to 0.40 microns, with the highest wear for the highest REA product. The mean dentine wear for all toothpaste products tested was positively correlated with RDA value (R2 = 0.897). The correlation was further improved if median wear values were used (R2 = 0.930). CONCLUSIONS This study has demonstrated a rapid technique for the simultaneous investigation of the abrasive wear of toothpaste on enamel and dentine. There was a good correlation between median dentine wear and RDA values.