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Biochemical methods confirmed the presence of the heparin-ATP complex. Acid sulfonate and carboxyl groups proved to mediate heparin-ATP complex formation. The technique for preparation of the heparin complex at a 1 : 20 weight ratio of the components and different pH was developed. Intramuscular administration of resulting complex increased anticoagulant and fibrinolytic activities of animal (rat) plasma. The obtained data indicate the presence of physiologically active heparin-ATP complex providing for anticoagulant effects in the body.
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