Biomaterial Database

The effects of saturated fatty acids on endothelial cells. 1992

C L Zhang, and V Lyngmo, and A Nordøy

Department of Medicine, University of Tromsø, Norway.

The present study reports on the effects of myristic(14:0), palmitic(16:0) and stearic(18:0) acids separately and combined with linoleic(18:2n-6), arachidonic(20:4n-6) and eicosapentaenoic(20:5n-3) acids on cultured human endothelial cell monolayers(ECM). The cell viability determined by trypan blue exclusion study, the release of lactate dehydrogenase(LDH) and 6-keto-PGF1 alpha into the medium from ECM incubated with albumin bound fatty acids(FA) were measured. The cell viability was reduced and the release of LDH from ECM was enhanced by 18:0(0.2mM, ratio 1, 2 and 4) and 16:0(0.4mM, ratio 2), but not by 14:0, or the unsaturated FA. The reduced cell viability induced by 18:0 was completely inhibited by polyunsaturated FA, 18:2n-6, 20:4n-6 and 20:5n-3. The release of 6-keto-PGF1 alpha was increased by 18:0, but not by 14:0 and 16:0. All three saturated FA potentiated 6-keto-PGF1 alpha production induced by 20:4n-6. Combined with 18:2n-6 or 20:5n-3, they also stimulated 6-keto-PGF1 alpha production. This study indicates that the three main dietary SFA, 18:0, 16:0 and 14:0 do not have the same effects on ECM. The damaging effect on endothelial cells induced by 18:0 and 16:0 may be of significance for the thrombogenic effects related to these saturated fatty acids.

UI	MeSH Term	Description	Entries
D007770	L-Lactate Dehydrogenase	A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.	Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D011464	Epoprostenol	A prostaglandin that is a powerful vasodilator and inhibits platelet aggregation. It is biosynthesized enzymatically from PROSTAGLANDIN ENDOPEROXIDES in human vascular tissue. The sodium salt has been also used to treat primary pulmonary hypertension (HYPERTENSION, PULMONARY).	Prostacyclin,Prostaglandin I2,Epoprostanol,Epoprostenol Sodium,Epoprostenol Sodium Salt, (5Z,9alpha,11alpha,13E,15S)-Isomer,Flolan,Prostaglandin I(2),Veletri
D002470	Cell Survival	The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.	Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004730	Endothelium, Vascular	Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.	Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D005227	Fatty Acids	Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)	Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014343	Trypan Blue	A diazo-naphthalene sulfonate that is widely used as a stain.	Diamine Blue,Niagara Blue,VisionBlue,Blue, Diamine,Blue, Niagara,Blue, Trypan
D015121	6-Ketoprostaglandin F1 alpha	The physiologically active and stable hydrolysis product of EPOPROSTENOL. Found in nearly all mammalian tissue.	6-Keto-PGF1 alpha,6-Oxo-PGF1 alpha,6-Oxoprostaglandin F1 alpha,6 Ketoprostaglandin F1 alpha,6 Keto PGF1 alpha,6 Oxo PGF1 alpha,6 Oxoprostaglandin F1 alpha,F1 alpha, 6-Ketoprostaglandin,F1 alpha, 6-Oxoprostaglandin,alpha, 6-Keto-PGF1,alpha, 6-Ketoprostaglandin F1,alpha, 6-Oxo-PGF1,alpha, 6-Oxoprostaglandin F1