BIOMAT Database Logo BIOMAT Database Logo

Effects of ticlopidine on monoclonal anti-CD9 antibody-induced platelet aggregation and microparticle generation. 1992

S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.

We analyzed the effects of ticlopidine on platelet aggregation and on microparticle (MP) formation when platelets were exposed to a monoclonal anti-CD9 antibody (NNKY1-19) in vitro. Even when NNKY1-19-induced platelet aggregation was completely inhibited by preincubation with anti-GPIIb/IIIa antibody or Arg-Gly-Asp-Ser, or by using washed platelets from a Glanzmann's thrombasthenia patient, the formation of MP was still observed. Prostaglandin E1 and protein kinase C antagonists (H-7 and staurosporine) inhibited both NNKY1-19-induced aggregation and MP formation. Ticlopidine or aspirin plus apyrase scarcely affected NNKY1-19-induced platelet aggregation, except to prolong the lag time. However, ticlopidine significantly inhibited MP formation (p less than 0.01). These results suggest that ticlopidine inhibits NNKY1-19-induced MP formation by a different mechanism to that of the other antagonists, and that this mechanism is unrelated to the inhibition of platelet aggregation.

UI MeSH Term Description Entries
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010316 Particle Size Relating to the size of solids. Particle Sizes,Size, Particle,Sizes, Particle
D010974 Platelet Aggregation The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS. Aggregation, Platelet
D011108 Polymers Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS). Polymer
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D005557 Formaldehyde A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717) Formalin,Formol,Methanal,Oxomethane
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
Studies on the dual effects on platelets of a monoclonal antibody to CD9, and on the properties of platelet CD9.
September 1999, Thrombosis research,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
Monoclonal antibody to CD9 inhibits platelet-induced human endothelial cell proliferation.
August 2006, Molecules and cells,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
An anti-platelet activating factor antibody and its effects on platelet aggregation.
November 1993, Osaka city medical journal,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
Effects of ticlopidine on von Willebrand factor-mediated shear-induced platelet activation and aggregation.
November 2001, Platelets,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
[Effects of anti-platelet agents on shear-induced platelet aggregation].
June 1990, [Rinsho ketsueki] The Japanese journal of clinical hematology,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
[Mechanism of platelet activation induced by TP 82, a monoclonal antibody against CD9].
July 1990, Rinsho byori. The Japanese journal of clinical pathology,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
Effect of a new monoclonal anti-glycoprotein IX antibody, KMP-9, on high shear-induced platelet aggregation.
August 1997, Thrombosis and haemostasis,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
Monoclonal antibody-induced platelet aggregation does not require interaction between antibody and platelet Fc receptor.
January 1992, Haematologica,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
Anti-thrombotic action of ticlopidine, a new platelet aggregation inhibitor.
June 1978, Thrombosis research,
S Nomura, and H Nagata, and M Suzuki, and K Iwata, and T Kawakatsu, and H Kido, and T Fukuroi, and K Yamaguchi, and M Yanabu, and T Soga
Anti-CD9 monoclonal antibody activates p72syk in human platelets.
June 1995, The Journal of biological chemistry,
Copied contents to your clipboard!