BIOMAT Database Logo BIOMAT Database Logo

Expression of matrix metalloproteinase-9 associated with ets-1 proto-oncogene in rat tubulointerstitial cells. 2005

Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Department of Medicine, Tokyo Women's Medical University Daini Hospital, Arakawaku, Tokyo, Japan. takashi020304@yahoo.co.jp

BACKGROUND Ets-1 proto-oncogene exhibits multiple activities in the transcriptional regulation of numerous genes including metalloproteinase (MMP)-1, -3 and -9. MMPs play an important role in the remodelling of extracellular matrix in various renal diseases. However, the role of the Ets-1-MMP axis in advanced renal diseases is uncertain. In the present study, we investigated whether Ets-1 is involved in interleukin (IL)-1-mediated expression of MMPs in tubulointerstitial cells. METHODS Rat renal fibroblasts (NRK-49F) and tubular epithelial cells (NRK-52E) were cultured and allocated to an IL-1beta-treated group (10 ng/ml), a platelet-derived growth factor (PDGF)-BB-treated group (25 ng/ml) and a control group. Protein and mRNA were extracted after 1, 6, 12 and 24 h of treatment. Parallel flasks were treated with 2 muM ets-1 antisense oligodeoxynucleotides (ODNs) before exposure to IL-1beta. The expression of Ets-1 protein was evaluated by western blotting. The activities of MMPs were evaluated by gelatin zymography. The expression of ets-1 and/or MMP-9 mRNA was evaluated semiquantitatively by real-time reverse transcription-polymerase chain reaction (RT-PCR). RESULTS In NRK-49F cells, Ets-1 protein increased significantly by 6.8-fold at 6 h, and MMP-9 activity increased significantly by 9.9-fold at 12 h in the IL-1beta-treated group compared with controls. MMP-2 and -3 activities also increased significantly in the IL-1beta-treated group. In NRK-52E cells, Ets-1 protein was 3.1 times higher at 1 h, and the latent form of MMP-9 activity increased 3.4-fold at 6 h in the IL-1beta group compared with controls. However, MMP-2 or MMP-3 activities were not markedly altered by IL-1beta treatment compared with controls. When the cells were treated with ets-1 antisense ODNs before IL-1beta treatment, Ets-1 protein expression decreased at least 50%, and MMP-9 activity was clearly inhibited in both cells. We also confirmed that MMP-9 activity was upregulated on days 21 and 28 in renal cortex of rat crescentic glomerulonephritis. CONCLUSIONS The Ets-1 transcriptional factor may participate in IL-1beta-mediated MMP-9 expression in tubulointerstitial cells.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007684 Kidney Tubules Long convoluted tubules in the nephrons. They collect filtrate from blood passing through the KIDNEY GLOMERULUS and process this filtrate into URINE. Each renal tubule consists of a BOWMAN CAPSULE; PROXIMAL KIDNEY TUBULE; LOOP OF HENLE; DISTAL KIDNEY TUBULE; and KIDNEY COLLECTING DUCT leading to the central cavity of the kidney (KIDNEY PELVIS) that connects to the URETER. Kidney Tubule,Tubule, Kidney,Tubules, Kidney
D008297 Male Males
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D005921 Glomerulonephritis Inflammation of the renal glomeruli (KIDNEY GLOMERULUS) that can be classified by the type of glomerular injuries including antibody deposition, complement activation, cellular proliferation, and glomerulosclerosis. These structural and functional abnormalities usually lead to HEMATURIA; PROTEINURIA; HYPERTENSION; and RENAL INSUFFICIENCY. Bright Disease,Kidney Scarring,Glomerulonephritides,Scarring, Kidney
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions

Related Publications

Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Renal expression of proto-oncogene Ets-1 on matrix remodeling in experimental diabetic nephropathy.
September 2011, Acta histochemica,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
ets-1 and ets-2 proto-oncogene expression in human leukemia cells and cell lines.
January 1989, Nouvelle revue francaise d'hematologie,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Expression of the ets-1 proto-oncogene in melanocytic lesions.
August 2003, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Renal expression of the Ets-1 proto-oncogene during progression of rat crescentic glomerulonephritis.
December 2000, Journal of the American Society of Nephrology : JASN,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Expression of the ets-1 proto-oncogene in human thyroid tumor.
January 1999, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Expression of the ets-1 proto-oncogene in human pancreatic carcinoma.
February 1998, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Expression of the ets-1 proto-oncogene in human colorectal carcinoma.
May 2001, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Proto-oncogene Ets-1 and the kidney.
September 2001, Nephron,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Ets-1 upregulates matrix metalloproteinase-1 expression through extracellular matrix adhesion in vascular endothelial cells.
February 2002, Biochemical and biophysical research communications,
Takashi Naito, and Yoko Tanihata, and Hideki Nishimura, and Toshihisa Tanaka, and Chieko Higuchi, and Takashi Taguchi, and Tsutomu Sanaka
Expression of the Ets-1 proto-oncogene correlates with malignant potential in human astrocytic tumors.
June 1999, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
Copied contents to your clipboard!