Tetrahydrocurcumin in plasma and urine: quantitation by high performance liquid chromatography. 2005

Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
Cancer Prevention and Control Program, Moores UCSD Cancer Center, University of California, San Diego, CA, USA. dheath@ucsd.edu

Tetrahydrocurcumin (THC), one of the major metabolites of curcumin, exhibits many of the same physiologic and pharmacological activities as curcumin and in some systems may exert greater antioxidant activity than curcumin. However, evaluation of clinical efficacy is limited by lack of sensitive methods for quantifying intake/absorption in blood or urine. We have developed a sensitive high performance liquid chromatography (HPLC) analytical method for detection of THC in plasma and urine. The method involves extracting the THC from 0.2 mL samples with 95% ethyl acetate/5% methanol, and beta-17-estradiol acetate as an internal standard. Analysis with a reversed-phase C18 column and UV detection at 280 nm demonstrates linear performance from 0.050 to 6.0 microg/mL in plasma, and 0.060 to 6.0 microg/mL in urine. The coefficients of variation for intra- and inter-assays were each<8.6%. The average recovery of THC from plasma and urine was greater than 98.5%. These data demonstrate a rapid, sensitive and accurate method for HPLC quantification of THC in plasma and urine.

UI MeSH Term Description Entries
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D003474 Curcumin A yellow-orange dye obtained from tumeric, the powdered root of CURCUMA longa. It is used in the preparation of curcuma paper and the detection of boron. Curcumin appears to possess a spectrum of pharmacological properties, due primarily to its inhibitory effects on metabolic enzymes. 1,6-Heptadiene-3,5-dione, 1,7-bis(4-hydroxy-3-methoxyphenyl)-, (E,E)-,Curcumin Phytosome,Diferuloylmethane,Mervia,Turmeric Yellow,Phytosome, Curcumin,Yellow, Turmeric
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000284 Administration, Oral The giving of drugs, chemicals, or other substances by mouth. Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000894 Anti-Inflammatory Agents, Non-Steroidal Anti-inflammatory agents that are non-steroidal in nature. In addition to anti-inflammatory actions, they have analgesic, antipyretic, and platelet-inhibitory actions. They act by blocking the synthesis of prostaglandins by inhibiting cyclooxygenase, which converts arachidonic acid to cyclic endoperoxides, precursors of prostaglandins. Inhibition of prostaglandin synthesis accounts for their analgesic, antipyretic, and platelet-inhibitory actions; other mechanisms may contribute to their anti-inflammatory effects. Analgesics, Anti-Inflammatory,Aspirin-Like Agent,Aspirin-Like Agents,NSAID,Non-Steroidal Anti-Inflammatory Agent,Non-Steroidal Anti-Inflammatory Agents,Nonsteroidal Anti-Inflammatory Agent,Anti Inflammatory Agents, Nonsteroidal,Antiinflammatory Agents, Non Steroidal,Antiinflammatory Agents, Nonsteroidal,NSAIDs,Nonsteroidal Anti-Inflammatory Agents,Agent, Aspirin-Like,Agent, Non-Steroidal Anti-Inflammatory,Agent, Nonsteroidal Anti-Inflammatory,Anti-Inflammatory Agent, Non-Steroidal,Anti-Inflammatory Agent, Nonsteroidal,Anti-Inflammatory Analgesics,Aspirin Like Agent,Aspirin Like Agents,Non Steroidal Anti Inflammatory Agent,Non Steroidal Anti Inflammatory Agents,Nonsteroidal Anti Inflammatory Agent,Nonsteroidal Anti Inflammatory Agents,Nonsteroidal Antiinflammatory Agents

Related Publications

Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
August 1985, Journal of chromatography,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
November 1987, Journal of chromatography,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
November 1990, Journal of chromatography,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
February 1981, Journal of chromatography,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
March 1980, Journal of pharmaceutical sciences,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
November 1990, Journal of chromatography,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
February 1985, Journal of chromatography,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
October 1983, Journal of chromatography,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
August 1983, Journal of chromatography,
Dennis D Heath, and Milagros A Pruitt, and Dean E Brenner, and Aynun N Begum, and Sally A Frautschy, and Cheryl L Rock
April 1987, Journal of chromatography,
Copied contents to your clipboard!