-transparent.png){kind=logo}
We have recently demonstrated a novel cytotoxic effect of human platelets against Toxoplasma gondii and a role for thromboxane (TX) in this process (Yong et al., 1991). We now report on the spectrum of lipid mediators released by human platelets after interaction with T. gondii. In addition to TXB2, human platelets after incubation with T. gondii for 90 min released 12-hydroxyheptadecatrienoic acid (12-HHT), 12-hydroxyeicosatetraenoic acid (12-HETE), and an unidentified peak (UVmax 234 nm) as determined by reverse-phase high-performance liquid chromatography. Thermospray-liquid chromatography/mass spectrometry analysis and straight-phase HPLC identified the unknown peak as a mixture of 13-hydroxyoctadecadienoic acid (HODE) and 9-HODE. Radiolabeling studies with [14C]linoleic acid indicated that the platelets were the cellular source of the octadecanoids with 13-HODE (87.7%) greater than 9-HODE (12.3%). Inhibitor studies with indomethacin indicated that 13-HODE was a lipoxygenase product and 9-HODE was a cyclooxygenase product of linoleic acid. Thus, Toxoplasma-stimulated platelets release oxygenated products of both arachidonic acid and linoleic acid which may be important in the host response to T. gondii infection.
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
December 1992,
Parasitology,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
January 1997,
Advances in experimental medicine and biology,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
January 1996,
Biochemical and biophysical research communications,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
July 1987,
Biochemical and biophysical research communications,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
January 2015,
BioMed research international,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
February 1990,
Prostaglandins, leukotrienes, and essential fatty acids,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
February 2012,
Infection and immunity,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
October 1985,
Proceedings of the National Academy of Sciences of the United States of America,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
March 2005,
Journal of agricultural and food chemistry,
W R Henderson, and
M Rashed, and
E C Yong, and
T R Fritsche, and
G K Chiang
January 2022,
MicroRNA (Shariqah, United Arab Emirates),
