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In a previous study we have shown that Chinese hamster ovary (CHO) cells deficient in intact peroxisomes, lack the nonspecific lipid transfer protein (nsL-TP; sterol carrier protein 2) (van Heusden, G.P.H., Bos, K., Raetz, C.R.H. and Wirtz, K.W.A. (1990) J. Biol. Chem. 265, 4105-4110). The consequences of the absence of peroxisomes and of nsL-TP on intracellular cholesterol metabolism have been investigated in two peroxisome-deficient CHO cell lines (CHO-82 and CHO-78). Compared with wild-type cells (CHO-K1), the incorporation of [3H]acetate into cholesterol was 3-fold higher in the CHO-82 cells and 2-fold higher in the CHO-78 cells. In agreement with an increased synthesis of cholesterol, a 2-3-fold higher 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase activity was measured in both mutant cell lines. On the other hand, addition of low density lipoprotein (LDL), mevalonate (30 mM) or 25-hydroxycholesterol (2 micrograms/ml) to cells grown in lipoprotein-deficient serum, demonstrated that in both mutant cell lines the down-regulation of HMG-CoA reductase and of cholesterol synthesis were comparable to that in wild-type cells. These results strongly suggest that, in addition to down-regulation by LDL-derived cholesterol, mevalonate and 25-hydroxycholesterol, HMG-CoA reductase activity is under control of peroxisomes and/or nsL-TP.
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
November 1985,
Experimental cell research,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
September 1992,
The Journal of biological chemistry,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
June 1996,
Israel journal of medical sciences,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
March 1991,
Cancer research,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
May 2007,
The Journal of biological chemistry,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
January 1994,
Advances in experimental medicine and biology,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
January 1997,
British journal of cancer,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
April 1982,
The Journal of biological chemistry,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
July 1978,
Biochimica et biophysica acta,
G P van Heusden, and
J R van Beckhoven, and
R Thieringer, and
C R Raetz, and
K W Wirtz
January 1987,
Methods in enzymology,
