[Expression of osteoclast differentiation factor and intercellular adhesion molecule-1 of bone marrow mesenchymal stem cells enhanced with osteogenic differentiation]. 2005

Jun Wang, and Zhi-He Zhao, and Song-Jia Luo, and Yu-Bo Fan
Key Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.

OBJECTIVE To investgate whether the expression of osteoclast differentiation factor (ODF), intercellular adhesion molecule-1 (ICAM-1) depended on the stage of osteoblastic differentiation from rat bone marrow mesenchymal stem cells (BMSC). METHODS BMSC (4 passage) were selected for osteogenic differentiation by treated with osteogenic supplements (OS). Cells were harvested by day 0, 3, 6, 9, 12, 18 respectively after osteogenic inducement. In each experiment, control and OS-treated cells were processed in parallel. ODF and ICAM-1 mRNA were analyzed by semiquantitave RT-PCR assay. RESULTS Expression of ODF was enhanced with osteogenic differentiation guadully. whereas, expression of ICAM-1 was activated at OS-treated day 6, then keeping at a stable level. CONCLUSIONS This study indicated that BMSC undergoing osteogenic inducement was an ideal model for studying the differentiation and maturation of osteoblasts. During the early stage of differentiation along osteoblasts from stem cells to osteocytes, BMSC or osteoprogenitor react somewhat differently from osteoblasts, suggesting the ability of osteoblasts to regulating differentiation and maturation of osteoclasts have been improved with osteogenic culture.

UI MeSH Term Description Entries
D010006 Osteoblasts Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone. Osteoblast
D010010 Osteoclasts A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption. Odontoclasts,Cementoclast,Cementoclasts,Odontoclast,Osteoclast
D010012 Osteogenesis The process of bone formation. Histogenesis of bone including ossification. Bone Formation,Ossification, Physiologic,Endochondral Ossification,Ossification,Ossification, Physiological,Osteoclastogenesis,Physiologic Ossification,Endochondral Ossifications,Ossification, Endochondral,Ossifications,Ossifications, Endochondral,Osteoclastogeneses,Physiological Ossification
D001854 Bone Marrow Cells Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells. Bone Marrow Cell,Cell, Bone Marrow,Cells, Bone Marrow,Marrow Cell, Bone,Marrow Cells, Bone
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013234 Stem Cells Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells. Colony-Forming Units,Mother Cells,Progenitor Cells,Colony-Forming Unit,Cell, Mother,Cell, Progenitor,Cell, Stem,Cells, Mother,Cells, Progenitor,Cells, Stem,Colony Forming Unit,Colony Forming Units,Mother Cell,Progenitor Cell,Stem Cell
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D053245 RANK Ligand A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B and OSTEOPROTEGERIN. It plays an important role in regulating OSTEOCLAST differentiation and activation. Tumor Necrosis Factor Ligand Superfamily Member 11,CD254 Antigen,OPGL Protein,Osteoclast Differentiation Factor,Osteoprotegerin Ligand,RANKL Protein,Receptor Activator of Nuclear Factor-kappa B Ligand,Receptor Activator of Nuclear Factor-kappaB Ligand,TNF Superfamily, Member 11,TRANCE Protein,Tumor Necrosis Factor-Related Activation-Induced Cytokine,Antigen, CD254,Differentiation Factor, Osteoclast,Receptor Activator of Nuclear Factor kappa B Ligand,Receptor Activator of Nuclear Factor kappaB Ligand,Tumor Necrosis Factor Related Activation Induced Cytokine

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