Development of immunoassay for detection of meat and bone meal in animal feed. 2005

Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
Department of Nutritional Sciences, College of Human Environmental Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, USA. kimshin@okstate.edu

An immunoassay system was developed for efficient detection of prohibited meat and bone meal (MBM) in animal feed. Monoclonal antibodies (MAbs) were raised against bovine smooth muscle autoclaved at 130 degrees C for 20 min. Among the 1,500 supernatants of hybridoma cells screened, MAbs 3E1, 1G3, and 3E10 were selected and characterized in this study. The first set of MAbs produced, 3E1 and 1G3, had stronger reactivity against MBM than against smooth muscle that was heat treated at 90 degrees C for 10 min. However, reactivity gradually increased against smooth muscle that was autoclaved at 130 degrees C for up to 1 h. The enzyme-linked immunosorbent assay for detection of MBM in animal feed was optimized with the MAb 3E10 because of its superior performance. MAb 3E10 diluted to 100-fold was used to differentiate bovine MBM from that of other species in ingredients used for commercial animal feeds and could detect down to 0.05% MBM mixed in animal feed.

UI MeSH Term Description Entries
D008903 Minerals Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Mineral
D009130 Muscle, Smooth Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed) Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000821 Animal Feed Foodstuff used especially for domestic and laboratory animals, or livestock. Fodder,Animal Feeds,Feed, Animal,Feeds, Animal,Fodders
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal

Related Publications

Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
November 2001, Nature,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
June 2004, Journal of food protection,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
January 2006, Journal of AOAC International,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
January 2004, Journal of AOAC International,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
October 2003, Environmental science & technology,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
May 2016, Journal of agricultural and food chemistry,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
December 2004, Journal of food protection,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
January 2006, Foodborne pathogens and disease,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
August 2006, Journal of applied microbiology,
Shin-Hee Kim, and Tung-Shi Huang, and Thomas A Seymour, and Cheng-I Wei, and Stephen C Kempf, and C Roger Bridgman, and Dragan Momcilovic, and Roger A Clemens, and Haejung An
July 2009, Journal of food protection,
Copied contents to your clipboard!