This study was undertaken to compare the effects of 1% cholesterol (Ch) on some liver mineral concentrations in rats fed with corn oil (C) or olive oil (O). Male Sprague-Dawley rats (n = 4 per group) were fed AIN76A semi-purified diets containing either 5% corn oil or 5% olive oil replacing corn oil with or without 1% cholesterol for 21 days. The analysis of minerals: Phosphorous (P), Potassium (K), Magnesium (Mg), and Sulfur (S), of liver were conducted by inductively coupled plasma (ICP) spectroscopic method. In the C fed rats addition of 1% cholesterol produced significant increase in P concentrations but not K, Mg, and S concentrations. In contrast, in O fed rats, 1% Ch significantly decreased Mg and S concentrations. There was no significant change in K and P concentrations. In conclusion, this study describes the interactions between dietary oils and cholesterol on certain mineral concentrations in liver of rats. The results obtained may have clinical significance and nutritional significance in cardiovascular health.