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Identification of triterpenoid compounds of Centella asiatica by thin-layer chromatography and mass spectrometry. 2006

Mercè Bonfill, and Susana Mangas, and Rosa M Cusidó, and Lidia Osuna, and M Teresa Piñol, and Javier Palazón
Laboratorio de Fisiología vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagonal 643, E-08028 Barcelona, Spain. mbonfill@ub.edu

The identification of the four principal triterpenoid components of Centella asiatica has been achieved by TLC on silica gel plates and mass spectrometry, as a modification of the method described in the European Pharmacopoeia (5th edn). A combination of ethyl acetate and methanol as the mobile phase was found to be successful in separating these compounds from the rest of the main components of the extract. The spots were detected with anisaldehyde solution. The separated compounds were confirmed by MALDI -TOF mass spectrometry.

UI MeSH Term Description Entries
D002855 Chromatography, Thin Layer Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Thin-Layer,Thin Layer Chromatography,Chromatographies, Thin Layer,Chromatographies, Thin-Layer,Thin Layer Chromatographies,Thin-Layer Chromatographies,Thin-Layer Chromatography
D013058 Mass Spectrometry An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers. Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass
D013729 Terpenes A class of compounds composed of repeating 5-carbon units of HEMITERPENES. Isoprenoid,Terpene,Terpenoid,Isoprenoids,Terpenoids
D014315 Triterpenes A class of terpenes (the general formula C30H48) formed by the condensation of six isoprene units, equivalent to three terpene units. Triterpene,Triterpenoid,Triterpenoids
D053978 Pentacyclic Triterpenes Five-ring derivatives of dammarane having a chair-chair-chair-boat configuration. They include the lupanes, oleananes, amyrins, GLYCYRRHIZIC ACID, and soyasaponins. Adianane Derivatives,Adiananes,Baccharane Triterpenes,Fernane Derivatives,Fernanes,Gammacerane Derivatives,Gammaceranes,Hopane Derivatives,Hopanes,Hopenoid Derivatives,Pentacyclic Triterpenoids,Triterpenes, Pentacyclic,Triterpenes, Baccharane
D028041 Centella A plant of the family APIACEAE which is the source of asiatic acid and asiaticoside. Centella asiatica (L.) Urb. Gotu kola,Hydrocotyle,Hydrocotyle asiatica,Mandukaparni,Centella asiatica,Centella asiaticas,Centellas,Gotu kolas,Hydrocotyle asiaticas,Hydrocotyles,Mandukaparnus,asiatica, Centella,asiaticas, Hydrocotyle,kola, Gotu

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