Differential targeting and functional specialization of sodium channels in cultured cerebellar granule cells. 2005

Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
Laboratoire de Neurophysiologie Cellulaire, CNRS UMR 6150, IFR Jean Roche, Faculté de Médecine, Boulevard Pierre Dramard, 13916, Marseille Cedex 20, France.

The ion channel dynamics that underlie the complex firing patterns of cerebellar granule (CG) cells are still largely unknown. Here, we have characterized the subcellular localization and functional properties of Na+ channels that regulate the excitability of CG cells in culture. As evidenced by RT-PCR and immunocytochemical analysis, morphologically differentiated CG cells expressed Nav1.2 and Nav1.6, though both subunits appeared to be differentially regulated. Nav1.2 was localized at most axon initial segments (AIS) of CG cells from 8 days in vitro DIV 8 to DIV 15. At DIV 8, Nav1.6 was found uniformly throughout somata, dendrites and axons with occasional clustering in a subset of AIS. Accumulation of Nav1.6 at most AIS was evident by DIV 13-14, suggesting it is developmentally regulated at AIS. The specific contribution of these differentially distributed Na+ channels has been assessed using a combination of methods that allowed discrimination between functionally compartmentalized Na+ currents. In agreement with immunolocalization, we found that fast activating-fully inactivating Na+ currents predominate at the AIS membrane and in the somatic plasma membrane.

UI MeSH Term Description Entries
D008564 Membrane Potentials The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization). Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D009474 Neurons The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM. Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002531 Cerebellum The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills. Cerebella,Corpus Cerebelli,Parencephalon,Cerebellums,Parencephalons
D003712 Dendrites Extensions of the nerve cell body. They are short and branched and receive stimuli from other NEURONS. Dendrite
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001369 Axons Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body. Axon
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated

Related Publications

Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
May 2012, Journal of cellular physiology,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
October 1990, Bioscience reports,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
May 1994, Molecular pharmacology,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
November 2002, Annals of the New York Academy of Sciences,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
November 1997, Neuroscience,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
January 2016, Toxicon : official journal of the International Society on Toxinology,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
January 1987, Journal of neuroscience research,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
January 1997, Neurotoxicology,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
January 1995, The Journal of neuroscience : the official journal of the Society for Neuroscience,
Nancy Osorio, and Gisèle Alcaraz, and Françoise Padilla, and François Couraud, and Patrick Delmas, and Marcel Crest
July 1983, The Journal of physiology,
Copied contents to your clipboard!