Evaluation of immunoglobulin G subclass antibodies against recombinant Fasciola gigantica cathepsin L1 in an enzyme-linked immunosorbent assay for serodiagnosis of human fasciolosis. 2005

Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

A cystatin capture enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola gigantica cathepsin L1 antigen was developed to detect specific immunoglobulin G (IgG) subclass antibodies (IgG1, IgG2, IgG3, and IgG4) and was evaluated for its diagnostic potential for human fasciolosis. In an analysis of the sera of 13 patients infected with F. gigantica, 209 patients with other parasitic infections, 32 cholangiocarcinoma patients, and 42 healthy controls, the IgG4-ELISA gave the highest diagnostic values. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method based on the detection of IgG4 antibody were 100%, 99.3%, 99.3%, 86.7%, and 100%, respectively. The results revealed that restricting the ELISA to the detection of specific IgG4 antibody enhanced the specificity and accuracy for the serodiagnosis of human fasciolosis.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002403 Cathepsins A group of lysosomal proteinases or endopeptidases found in aqueous extracts of a variety of animal tissues. They function optimally within an acidic pH range. The cathepsins occur as a variety of enzyme subtypes including SERINE PROTEASES; ASPARTIC PROTEINASES; and CYSTEINE PROTEASES. Cathepsin
D003546 Cysteine Endopeptidases ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005209 Fasciola A genus of trematode liver flukes of the family Fasciolidae. Two species of this genus are F. hepatica and F. gigantica. The parasites are found in the liver and gallbladder and associated ducts in mammals and occasionally man. F. gigantica occurs rarely in man. Fasciolas
D005211 Fascioliasis Liver disease caused by infections with parasitic flukes of the genus FASCIOLA, such as FASCIOLA HEPATICA. Fasciola Infection,Fasciola Infections,Fascioliases,Infection, Fasciola,Infections, Fasciola
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
June 2021, Parasitology international,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
January 2022, PeerJ,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
September 2003, Acta tropica,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
May 2007, Clinical and vaccine immunology : CVI,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
September 1997, Clinical and diagnostic laboratory immunology,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
July 2007, Pakistan journal of biological sciences : PJBS,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
June 2020, Acta parasitologica,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
September 1996, The Southeast Asian journal of tropical medicine and public health,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
June 2008, Parasitology research,
Chaisiri Wongkham, and Chairat Tantrawatpan, and Pewpan M Intapan, and Wanchai Maleewong, and Sopit Wongkham, and Kunio Nakashima
December 2015, Journal of parasitic diseases : official organ of the Indian Society for Parasitology,
Copied contents to your clipboard!