Students investigating the antiproliferative effects of synthesized drugs on mouse mammary tumor cells. 2005

Rasha Hammamieh, and Margery Anderson, and Katharine Carr, and Christine N Tran, and Debra L Yourick, and Marti Jett
Division of Pathology, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA. rasha.hammamieh@na.amedd.army.mil

The potential for personalized cancer management has long intrigued experienced researchers as well as the naïve student intern. Personalized cancer treatments based on a tumor's genetic profile are now feasible and can reveal both the cells' susceptibility and resistance to chemotherapeutic agents. In a weeklong laboratory investigation that mirrors current cancer research, undergraduate and advanced high school students determine the efficacy of common pharmacological agents through in vitro testing. Using mouse mammary tumor cell cultures treated with "unknown" drugs historically recommended for breast cancer treatment, students are introduced to common molecular biology techniques from in vitro cell culture to fluorescence microscopy. Student understanding is assessed through laboratory reports and the successful identification of the unknown drug. The sequence of doing the experiment, applying logic, and constructing a hypothesis gives the students time to discover the rationale behind the cellular drug resistance assay. The breast cancer experiment has been field tested during the past 5 yr with more than 200 precollege/undergraduate interns through the Gains in the Education of Mathematics and Science program hosted by the Walter Reed Army Institute of Research.

UI MeSH Term Description Entries
D003479 Curriculum A course of study offered by an educational institution. Curricula,Short-Term Courses,Course, Short-Term,Courses, Short-Term,Short Term Courses,Short-Term Course
D004354 Drug Screening Assays, Antitumor Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals. Anticancer Drug Sensitivity Tests,Antitumor Drug Screens,Cancer Drug Tests,Drug Screening Tests, Tumor-Specific,Dye Exclusion Assays, Antitumor,Anti-Cancer Drug Screens,Antitumor Drug Screening Assays,Tumor-Specific Drug Screening Tests,Anti Cancer Drug Screens,Anti-Cancer Drug Screen,Antitumor Drug Screen,Cancer Drug Test,Drug Screen, Anti-Cancer,Drug Screen, Antitumor,Drug Screening Tests, Tumor Specific,Drug Screens, Anti-Cancer,Drug Screens, Antitumor,Drug Test, Cancer,Drug Tests, Cancer,Screen, Anti-Cancer Drug,Screen, Antitumor Drug,Screens, Anti-Cancer Drug,Screens, Antitumor Drug,Test, Cancer Drug,Tests, Cancer Drug,Tumor Specific Drug Screening Tests
D004521 Educational Measurement The assessing of academic or educational achievement. It includes all aspects of testing and test construction. Assessment, Educational,Graduate Records Examination,Educational Assessment,Examination, Graduate Records,Measurement, Educational,Assessments, Educational,Educational Assessments,Educational Measurements,Examinations, Graduate Records,Graduate Records Examinations,Measurements, Educational
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000970 Antineoplastic Agents Substances that inhibit or prevent the proliferation of NEOPLASMS. Anticancer Agent,Antineoplastic,Antineoplastic Agent,Antineoplastic Drug,Antitumor Agent,Antitumor Drug,Cancer Chemotherapy Agent,Cancer Chemotherapy Drug,Anticancer Agents,Antineoplastic Drugs,Antineoplastics,Antitumor Agents,Antitumor Drugs,Cancer Chemotherapy Agents,Cancer Chemotherapy Drugs,Chemotherapeutic Anticancer Agents,Chemotherapeutic Anticancer Drug,Agent, Anticancer,Agent, Antineoplastic,Agent, Antitumor,Agent, Cancer Chemotherapy,Agents, Anticancer,Agents, Antineoplastic,Agents, Antitumor,Agents, Cancer Chemotherapy,Agents, Chemotherapeutic Anticancer,Chemotherapy Agent, Cancer,Chemotherapy Agents, Cancer,Chemotherapy Drug, Cancer,Chemotherapy Drugs, Cancer,Drug, Antineoplastic,Drug, Antitumor,Drug, Cancer Chemotherapy,Drug, Chemotherapeutic Anticancer,Drugs, Antineoplastic,Drugs, Antitumor,Drugs, Cancer Chemotherapy
D001695 Biology One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.
D013334 Students Individuals enrolled in a school or formal educational program. School Enrollment,Enrollment, School,Enrollments, School,School Enrollments,Student
D013663 Teaching A formal and organized process of transmitting knowledge to a person or group. Teaching Method,Training Activity,Training Technique,Academic Training,Educational Technics,Educational Techniques,Pedagogy,Teaching Methods,Technics, Educational,Techniques, Educational,Training Activities,Training Technics,Training Techniques,Activities, Training,Activity, Training,Educational Technic,Educational Technique,Method, Teaching,Methods, Teaching,Pedagogies,Technic, Educational,Technic, Training,Technics, Training,Technique, Educational,Technique, Training,Techniques, Training,Training Technic,Training, Academic
D014495 Universities Educational institutions providing facilities for teaching and research and authorized to grant academic degrees. University

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