-transparent.png){kind=logo}
Conformational changes of Escherichia coli sigma54-RNA-polymerase upon closed-promoter complex formation. 2005
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
Department of Biological Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
RNA polymerase from the mesophile Escherichia coli exists in two forms, the core enzyme and the holoenzyme. Using cryo-electron microscopy and single-particle analysis, we have obtained the structure of the complete RNA polymerase from E.coli containing the sigma54 factor within the closed-promoter complex. Comparisons with earlier reconstructions of the core enzyme and the sigma54 holoenzyme reveal the behaviour of this major variant RNA polymerase in defined functional states. The binding of DNA leads to significant conformational changes in the enzyme's catalytic subunits, apparently a necessity for the initiation of enhancer-dependent promoter-specific transcription.
Related Publications
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
December 1987,
Biochimica et biophysica acta,
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
December 1993,
Nucleic acids research,
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
July 1979,
FEBS letters,
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
October 2021,
Proceedings of the National Academy of Sciences of the United States of America,
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
June 1989,
Journal of molecular biology,
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
September 2008,
EcoSal Plus,
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
May 2001,
Genes to cells : devoted to molecular & cellular mechanisms,
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
December 1998,
Biochemistry,
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
January 2009,
Methods (San Diego, Calif.),
Pampa Ray, and
Richard J Hall, and
Robert D Finn, and
Shaoxia Chen, and
Ardan Patwardhan, and
Martin Buck, and
Marin van Heel
May 1985,
Biochemistry,
