High-performance liquid chromatographic determination of vertilmicin in rat plasma using sensitive fluorometric derivatization. 2005

Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
Department of Pharmaceutical Analysis, School of Pharmacy, Medical Center of Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, PR China.

A sensitive and reliable high-performance liquid chromatographic method was developed for the determination of vertilmicin in rat plasma. Derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) followed by C(18) reversed-phase chromatography allowed the fluorimetric detection of vertilmicin. Optimal conditions for the derivatization of vertilmicin are described. The limit of quantification was 0.02 mg/L. The pharmacokinetics of vertilmicin was studied in 24 rats following intramuscular injection (i.m.) of different doses (4, 8, 16, 32 mg/kg of body weight). The pharmacokinetic parameter values were estimated by use of 3P97 program. In this study, we assessed the dose proportionality of vertilmicin after single intramuscular injection doses and obtained new information on the pharmacokinetics of the compound.

UI MeSH Term Description Entries
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005449 Fluorenes A family of diphenylenemethane derivatives.
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000617 Aminoglycosides Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS. Aminoglycoside
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
February 2011, Journal of separation science,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
December 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
November 1985, Clinical chemistry,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
March 1986, Journal of chromatography,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
January 1994, Journal of chromatography,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
October 1996, Journal of chromatography. B, Biomedical applications,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
June 1996, Journal of pharmaceutical and biomedical analysis,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
November 1991, Pharmaceutical research,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
January 2002, Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques,
Zhen Liu, and Yunfei Sha, and Taomin Huang, and Bei Yang, and Geng-Li Duan
February 1993, Therapeutic drug monitoring,
Copied contents to your clipboard!