Interaction of bacterial endotoxine (lipopolysaccharide) with latex particles: application to latex agglutination immunoassays. 2002

J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
Departamento de Física Aplicada II, Universidad de Málaga, Málaga, 29071, Spain.

The latex agglutination immunoassay technique uses polymer colloids as carriers for antibodies or antigens to enhance the immunological reaction. In this work, the interaction of a lipopolysaccharide (LPS) of Brucella Melitensis with two conventional latexes has been studied. Some experiments on the physical adsorption of the LPS onto these polystyrene beads have been performed and several complexes with different coverage degrees were obtained by modifying the incubation conditions. Regarding the application in the development of diagnostic test systems, it is advisable to study the latex-LPS complexes from an electrokinetic and colloidal stability point of view. The complexes were electrokinetically characterized by measuring the electrophoretic mobility under different redispersion conditions. The colloidal stability was determined by simple turbidity measurements. Experimental and theoretical data have been employed to study the molecular disposition of the LPS in the latex particle surface to compare with the outer membrane of bacterial cells. Latex complexes covered by different LPS amounts showed high colloidal stability and adequate immunoreactivity that remains for a long time period.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D007840 Latex A milky, product excreted from the latex canals of a variety of plant species that contain cauotchouc. Latex is composed of 25-35% caoutchouc, 60-75% water, 2% protein, 2% resin, 1.5% sugar & 1% ash. RUBBER is made by the removal of water from latex.(From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed). Hevein proteins are responsible for LATEX HYPERSENSITIVITY. Latexes are used as inert vehicles to carry antibodies or antigens in LATEX FIXATION TESTS. Latices,Latice
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D008863 Microspheres Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers. Latex Beads,Latex Particles,Latex Spheres,Microbeads,Bead, Latex,Beads, Latex,Latex Bead,Latex Particle,Latex Sphere,Microbead,Microsphere,Particle, Latex,Particles, Latex,Sphere, Latex,Spheres, Latex
D010316 Particle Size Relating to the size of solids. Particle Sizes,Size, Particle,Sizes, Particle
D011137 Polystyrenes Polymerized forms of styrene used as a biocompatible material, especially in dentistry. They are thermoplastic and are used as insulators, for injection molding and casting, as sheets, plates, rods, rigid forms and beads. Polystyrol,Polystyrene,Polystyrols
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000327 Adsorption The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily. Adsorptions
D000372 Agglutination Tests Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed) Agglutination Test,Test, Agglutination,Tests, Agglutination

Related Publications

J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
January 1997, Journal of biomaterials science. Polymer edition,
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
May 1965, Journal of immunology (Baltimore, Md. : 1950),
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
April 1965, Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists,
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
August 1984, Iyo denshi to seitai kogaku. Japanese journal of medical electronics and biological engineering,
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
August 1995, Archives of disease in childhood,
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
December 1990, Journal of immunological methods,
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
September 1963, American journal of clinical pathology,
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
January 1983, Biomedica biochimica acta,
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
August 1991, The Tohoku journal of experimental medicine,
J M Peula-García, and J A Molina-Bolivar, and J Velasco, and A Rojas, and F Galisteo-González
January 1962, Vox sanguinis,
Copied contents to your clipboard!