BIOMAT Database Logo BIOMAT Database Logo

Absolute quantitation of viremia in human immunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction. 1992

S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Institute of Microbiology, University of Ancona, Italy.

A competitive polymerase chain reaction (PCR)-based assay for the quantitative detection of human immunodeficiency virus type 1 (HIV-1) viremia was developed and optimized. This method consists of the reverse transcription and subsequent amplification in the same tube of two similar RNA templates, the wild-type template to be quantified and a known amount of the internally deleted synthetic template, both with identical primer recognition sites. The same strategy also proved to be useful in the quantitative assay of HIV-1-specific cellular transcripts and proviral DNA sequences from peripheral blood mononuclear cells by using competitor DNA. The method might be of interest in the study of the precise level of HIV-1 activity during the different clinical phases of the infection and in the simple, fast, and methodologically correct molecular investigation of patients treated with specific antiviral compounds.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014158 Transcription, Genetic The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. Genetic Transcription
D014766 Viremia The presence of viruses in the blood. Viremias
D015497 HIV-1 The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte. Human immunodeficiency virus 1,HIV-I,Human Immunodeficiency Virus Type 1,Immunodeficiency Virus Type 1, Human
D015658 HIV Infections Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS). HTLV-III Infections,HTLV-III-LAV Infections,T-Lymphotropic Virus Type III Infections, Human,HIV Coinfection,Coinfection, HIV,Coinfections, HIV,HIV Coinfections,HIV Infection,HTLV III Infections,HTLV III LAV Infections,HTLV-III Infection,HTLV-III-LAV Infection,Infection, HIV,Infection, HTLV-III,Infection, HTLV-III-LAV,Infections, HIV,Infections, HTLV-III,Infections, HTLV-III-LAV,T Lymphotropic Virus Type III Infections, Human
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain

Related Publications

S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Competitive reverse transcription-polymerase chain reaction for quantitation of feline immunodeficiency virus.
April 1995, Journal of virological methods,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Quantitation of Angiotensin II Receptors by Competitive Reverse-Transcription Polymerase Chain Reaction.
January 2001, Methods in molecular medicine,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Quantitation of plasma human immunodeficiency virus type 1 RNA by competitive polymerase chain reaction.
June 1992, The Journal of infectious diseases,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Quantitation of equine cytokine mRNA expression by reverse transcription-competitive polymerase chain reaction.
January 1999, Veterinary immunology and immunopathology,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Quantitation of hepatitis C viraemia by a competitive reverse transcription-polymerase chain reaction system.
January 1996, Annals of the Academy of Medicine, Singapore,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Quantitation of beta 1 triiodothyronine receptor mRNA in human tissues by competitive reverse transcription polymerase chain reaction.
November 1995, Journal of endocrinological investigation,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Quantitation of bovine TNF-alpha mRNA by reverse transcription and competitive polymerase chain reaction amplification.
January 1995, Veterinary immunology and immunopathology,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Competitive reverse transcription-polymerase chain reaction for quantifying murine AIDS virus.
December 1996, Journal of virological methods,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction.
January 1995, Research in virology,
S Menzo, and P Bagnarelli, and M Giacca, and A Manzin, and P E Varaldo, and M Clementi
Detection of feline immunodeficiency virus transcripts by quantitative reverse transcription-polymerase chain reaction.
February 1996, Veterinary microbiology,
Copied contents to your clipboard!