Column-switching high-performance liquid chromatographic method for the determination of zaltoprofen in rat plasma. 2006

Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
Division of Biopharmaceutics, Department of Pharmacology, National Institute of Toxicological Research, Korea Food and Drug Administration, Nokbun-dong 5, Eunpyung-Ku, Seoul, South Korea. sochoi@kfda.go.kr

A direct injection column-switching high-performance liquid chromatography (HPLC) method was developed and validated for quantification of zaltoprofen in rat plasma. Following dilution with mobile phase A, i.e. acetonitrile-10mM potassium phosphate buffer (pH 6.8) (12:88, v/v) samples were directly injected to the pre-column without sample pre-purification step. After endogenous plasma components were eluted to waste, the system was switched and the analyte was eluted to the trap column. Zaltoprofen was then back-flushed to the analytical column for separation with mobile phase B, i.e. acetonitrile-10mM potassium phosphate buffer (pH 6.8) (35:65, v/v) and quantification with an ultraviolet detector at 230 nm. The calibration curve was linear in the concentration range of 40-5000 ngmL(-1). This method has been fully validated and shown to be specific, accurate and precise. The method is simple, rapid and the sample preparation is minimal and appears to be useful for the pharmacokinetic study of zaltoprofen.

UI MeSH Term Description Entries
D008297 Male Males
D011422 Propionates Derivatives of propionic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxyethane structure. Propanoate,Propanoic Acid,Propionate,Propanoates,Propanoic Acid Derivatives,Propanoic Acids,Propionic Acid Derivatives,Propionic Acids,Acid, Propanoic,Acids, Propanoic,Acids, Propionic,Derivatives, Propanoic Acid,Derivatives, Propionic Acid
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004355 Drug Stability The chemical and physical integrity of a pharmaceutical product. Drug Shelf Life,Drugs Shelf Lives,Shelf Life, Drugs,Drug Stabilities,Drugs Shelf Life,Drugs Shelf Live,Life, Drugs Shelf,Shelf Life, Drug,Shelf Live, Drugs,Shelf Lives, Drugs
D000284 Administration, Oral The giving of drugs, chemicals, or other substances by mouth. Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000894 Anti-Inflammatory Agents, Non-Steroidal Anti-inflammatory agents that are non-steroidal in nature. In addition to anti-inflammatory actions, they have analgesic, antipyretic, and platelet-inhibitory actions. They act by blocking the synthesis of prostaglandins by inhibiting cyclooxygenase, which converts arachidonic acid to cyclic endoperoxides, precursors of prostaglandins. Inhibition of prostaglandin synthesis accounts for their analgesic, antipyretic, and platelet-inhibitory actions; other mechanisms may contribute to their anti-inflammatory effects. Analgesics, Anti-Inflammatory,Aspirin-Like Agent,Aspirin-Like Agents,NSAID,Non-Steroidal Anti-Inflammatory Agent,Non-Steroidal Anti-Inflammatory Agents,Nonsteroidal Anti-Inflammatory Agent,Anti Inflammatory Agents, Nonsteroidal,Antiinflammatory Agents, Non Steroidal,Antiinflammatory Agents, Nonsteroidal,NSAIDs,Nonsteroidal Anti-Inflammatory Agents,Agent, Aspirin-Like,Agent, Non-Steroidal Anti-Inflammatory,Agent, Nonsteroidal Anti-Inflammatory,Anti-Inflammatory Agent, Non-Steroidal,Anti-Inflammatory Agent, Nonsteroidal,Anti-Inflammatory Analgesics,Aspirin Like Agent,Aspirin Like Agents,Non Steroidal Anti Inflammatory Agent,Non Steroidal Anti Inflammatory Agents,Nonsteroidal Anti Inflammatory Agent,Nonsteroidal Anti Inflammatory Agents,Nonsteroidal Antiinflammatory Agents
D001578 Benzopyrans Compounds with a core of fused benzo-pyran rings. Benzopyran,Chromene,Chromenes
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats

Related Publications

Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
July 1992, Journal of chromatography,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
April 2001, Journal of chromatography. B, Biomedical sciences and applications,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
March 2005, Journal of pharmaceutical and biomedical analysis,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
December 1990, Pharmaceutical research,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
June 2000, Journal of chromatography. B, Biomedical sciences and applications,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
February 1984, Journal of chromatography,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
July 1993, Journal of chromatography,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
March 2004, Clinical biochemistry,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
June 1994, Therapeutic drug monitoring,
Sun Ok Choi, and So Young Um, and Sung Hee Jung, and Seo Jeong Jung, and Joo Il Kim, and Hwa Jeong Lee, and Soo Youn Chung
April 2001, Talanta,
Copied contents to your clipboard!