Biomaterial Database

Activation of Rac1 by RhoG regulates cell migration. 2006

Hironori Katoh, and Kiyo Hiramoto, and Manabu Negishi

Laboratory of Molecular Neurobiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. hirokato@pharm.kyoto-u.ac.jp

Cell migration is essential for normal development and many pathological processes. Rho-family small GTPases play important roles in this event. In particular, Rac regulates lamellipodia formation at the leading edge during migration. The small GTPase RhoG activates Rac through its effector ELMO and the ELMO-binding protein Dock180, which functions as a Rac-specific guanine nucleotide exchange factor. Here we investigated the role of RhoG in cell migration. RNA interference-mediated knockdown of RhoG in HeLa cells reduced cell migration in Transwell and scratch-wound migration assays. In RhoG-knockdown cells, activation of Rac1 and formation of lamellipodia at the leading edge in response to wounding were attenuated. By contrast, expression of active RhoG promoted cell migration through ELMO and Dock180. However, the interaction of Dock180 with Crk was dispensable for the activation of Rac1 and promotion of cell migration by RhoG. Taken together, these results suggest that RhoG contributes to the regulation of Rac activity in migrating cells.

UI	MeSH Term	Description	Entries
D011993	Recombinant Fusion Proteins	Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.	Fusion Proteins, Recombinant,Recombinant Chimeric Protein,Recombinant Fusion Protein,Recombinant Hybrid Protein,Chimeric Proteins, Recombinant,Hybrid Proteins, Recombinant,Recombinant Chimeric Proteins,Recombinant Hybrid Proteins,Chimeric Protein, Recombinant,Fusion Protein, Recombinant,Hybrid Protein, Recombinant,Protein, Recombinant Chimeric,Protein, Recombinant Fusion,Protein, Recombinant Hybrid,Proteins, Recombinant Chimeric,Proteins, Recombinant Fusion,Proteins, Recombinant Hybrid
D002465	Cell Movement	The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.	Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D004789	Enzyme Activation	Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.	Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D006367	HeLa Cells	The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays.	Cell, HeLa,Cells, HeLa,HeLa Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015398	Signal Transduction	The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.	Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal
D048868	Adaptor Proteins, Signal Transducing	A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymes	Signal Transducing Adaptor Proteins
D050737	Proto-Oncogene Proteins c-crk	Signal transducing adaptor proteins that contain SRC HOMOLOGY DOMAINS and play a role in CYTOSKELETON reorganization. c-crk protein is closely related to ONCOGENE PROTEIN V-CRK and includes several alternatively spliced isoforms.	c-crk Protein,crk Proto-Oncogene Proteins,CRKI Protein,CRKII Protein,CT10 Regulator of Kinase Protein,Crk-1 Protein,Crk-2 Protein,Crk3 Protein,CrkII Adapter Protein,CrkIII Protein,Proto-Oncogene Protein c-crk,Proto-Oncogene Protein c-crk-1,Proto-Oncogene Protein c-crk-2,Proto-Oncogene Protein c-crk-3,c-CrkII Protein,c-CrkIII Protein,c-crk Proto-Oncogene Proteins,c-crk-2 Proto-Oncogene Protein,c-crk-3 Proto-Oncogene Protein,c-crk-I Proto-Oncogene Protein,c-crkI Protein,Adapter Protein, CrkII,Crk 1 Protein,Crk 2 Protein,Proteins c-crk, Proto-Oncogene,Proto Oncogene Protein c crk,Proto Oncogene Protein c crk 1,Proto Oncogene Protein c crk 2,Proto Oncogene Protein c crk 3,Proto Oncogene Proteins c crk,Proto-Oncogene Protein, c-crk-2,Proto-Oncogene Protein, c-crk-3,Proto-Oncogene Protein, c-crk-I,Proto-Oncogene Proteins, c-crk,Proto-Oncogene Proteins, crk,c CrkII Protein,c CrkIII Protein,c crk 2 Proto Oncogene Protein,c crk 3 Proto Oncogene Protein,c crk I Proto Oncogene Protein,c crk Proto Oncogene Proteins,c-crk, Proto-Oncogene Protein,c-crk, Proto-Oncogene Proteins,c-crk-1, Proto-Oncogene Protein,c-crk-2, Proto-Oncogene Protein,c-crk-3, Proto-Oncogene Protein,crk Proto Oncogene Proteins
D020741	rho GTP-Binding Proteins	A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47.	rho G-Proteins,rho GTPase,rho GTPases,rho Small GTP-Binding Proteins,P21 (rho)Protein,rho GTP-Binding Protein,rho Protein P21,G-Proteins, rho,GTP-Binding Protein, rho,GTP-Binding Proteins, rho,GTPase, rho,GTPases, rho,P21, rho Protein,rho G Proteins,rho GTP Binding Protein,rho GTP Binding Proteins,rho Small GTP Binding Proteins